MORPHOLOGICAL AND MOLECULAR-BIOLOGICAL STUDIES ON STRUCTURE AND PHYLOGENY OF CHLOROPLAST OF A GREEN DINOFLAGELLATE.

绿色甲藻叶绿体结构和系统发育的形态学和分子生物学研究。

• 批准号: 04640645
• 负责人: WATANABE Makoto M.
• 金额: $ 1.02万
• 依托单位: NATIONAL INSTITUTE FOR ENVIRONMENTAL STUDIES
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04640645/
• 关键词: GREEN DINOFLAGELLATE; LEPIDODINIUM VIRIDE; NUCLEOMORPH; FLAGELLAR APPARATUS; TANSVERSE STRIATED ROOT; LOMGITUDINAL MICROTUBULAR ROOT; CHLOROPLAST ssu rRNA; Lepidodiniun viride; 葉緑体; DNA; ヌクレオモルフ; プライマー

Immunogold electron microscopy of DNA distribution in chloroplasts of a green dinoflagellate was carried out in order to detect DNA in nucleomorph-like, double-membrane bound vesicle in chloroplasts. As a result, immunogold-labeled materials were detected in the double-membranebound vesicle. It reveals that nucleomorph derived from endosymbiont is present in chloroplasts.The ultrastructure of flagellar apparatus of the host dinoflagellate was observed in order to better understand phylogenetic relationship between the green dinoflagaellate, Lepidodinium viride and the other dinoflgaellates. L.viride had the transverse striated root (TSR) and the posteriorly directed longitudinal microtubular root (LSR) which are tne most cammonly reported flagellar apparatus of dinoflagellates. The basal bodies of L.vreide lay at an angle of approximately 120-130ﾟ which has been observed in all the gymnodinioid dinoflagellates. The most unusual features of the flagellar apparatus of L.viride are the cu … More rved microtubular root (CMR) located in the dorsal portion of the transverse bassal body and the longitudinal striated root connective (LSR) which originates from th proximal end of longitudinal basal body and extends along the ventral-right edge of the LMR.These apparatus have never been reported in the other dinoflagellates. In addition, the LMR bent at nearly 90ﾟ at the proximal ends of basal bodies and a narrow striated fibrous connective linked the posterior part of the LMR with the microtubular bundle lying along the longitudinal flagellar canal and directing to the peduncle-like cytoplasmic projection. These features have never been detected in the other dinoflagellates. Conclusively, these results support the establishment of the genus Lepidodinium.The chloroplast small subunit (ssu) ribosomal (r) RNA gene of a green alga Chlamydomonas parkeae was amplified by polymerase chain reaction (PCR), sequenced and compared with those of the typical volvocalean algae and the prasinophycean alga Pyramimonas parkeae in order to get the basic knowledge for analysis on phylogeny of the chloroplasts of a green dinofllagellate, L.viride. As a result, Chlamydomonas and Pyramimonas were assignable to the different groups from each other. Based on the knowledge obtained, the phylogeny of the chloroplasts of L.viride can be demonstrated by sequencing the chloroplast ssu rRNA of L.viride and comparing it with the present results. Less

为了检测叶绿体中核形态样双膜结合囊泡中的 DNA，对绿色甲藻叶绿体中的 DNA 分布进行了免疫金电子显微镜检查，结果在双膜结合囊泡中检测到了免疫金标记物质。它揭示了叶绿体中存在源自内共生体的核形态。鞭毛的超微结构为了更好地了解绿色甲藻、Lepidodinium v​​iride 和其他甲藻之间的系统发育关系，对宿主甲藻的器官进行了观察。最常见报道的甲藻基部鞭毛器 L.vreide 的身体以大约 30° 的角度放置。 120-130ﾟ，已在所有裸甲藻中观察到。L.viride 鞭毛器最不寻常的特征是位于横向基底体的背侧部分和纵向的弯曲微管根（CMR）。横纹根结缔组织（LSR），起源于纵向基体的近端，并沿着 LMR 的腹侧右边缘延伸。这些装置从未被此外，LMR 在基体近端弯曲近 90°，并且狭窄的条纹纤维结缔组织将 LMR 的后部与沿着纵向鞭毛管并指向鞭毛管的微管束连接起来。这些特征从未在其他甲藻中检测到。最终，这些结果支持该属的建立。通过聚合酶链式反应 (PCR) 扩增绿藻 Chlamydomonas Parkeae 的叶绿体小亚基 (ssu) 核糖体 (r) RNA 基因，测序并与典型的藻类和 prasinophycean 藻 Pyramimonas Parkeae 的基因进行比较，以便获得分析绿色植物叶绿体系统发育的基本知识结果，衣藻属和锥体藻属彼此被分配到不同的组，基于所获得的知识，可以通过对 L.viride 的叶绿体 ssu rRNA 进行测序来证明 L.viride 的叶绿体的系统发育。 viride 并将其与当前结果进行比较。

项目成果

M.M.Watanabe,S.Suda and I.Inouye: "Flagellar apparatus of a greendinoflagellate, Lepidodinium viride(oinophyfa)" Jpn.J.Phycol.42(予定). (1994)

M.M.Watanabe、S.Suda 和 I.Inouye：“绿鞭毛虫的鞭毛装置，Lepidodinium v​​iride（oinophyfa）” Jpn.J.Phycol.42（计划）（1994 年）。

M.M.Watanabe,S.Suda and I.Inouye: "Flagellar apparatus of a green dinotlagellate,Lepidodinium viride(Dinophyta)" Jpn.J.Phycol.42(発表予定). (1994)

M.M.Watanabe、S.Suda 和 I.Inouye：“绿色甲藻的鞭毛装置，Lepidodinium v​​iride（Dinophyta）” Jpn.J.Phycol.42（待出版）（1994 年）。

Y-S.Kim,H.Oyaizu,S.Matsumoto.M,M.Watanabe,and H.Nozaki: "Phylogeny of the pealiar prgment-containing narine flagellate Chlamydomonas parkeae(Chlorophyta)inferred from the chloroplast small oubunit ribosomal RNA gene sequences." Phycologia. 33(発表予定). (1994

Y-S.Kim、H.Oyaizu、S.Matsumoto.M、M.Watanabe 和 H.Nozaki：“从叶绿体小单位核糖体 RNA 基因序列推断出含有 paliar 片段的 narine 鞭毛藻 Chlamydomonas Parkeae（绿藻门）的系统发育。”藻类学。33（待提交）。

M.M.Watanabe, S.Suda and I.Inouye: "Flagellar apparatus of a green dinoflagellate, Lepidodinium viride (Dinophyta)." Jpn J.Phycol.42 in press.(1994)

M.M.Watanabe、S.Suda 和 I.Inouye：“绿色甲藻的鞭毛器，Lepidodinium v​​iride（甲藻门）。”

Y.S Kim,H.Oyaizu,S.Matsumoto,M.M.Watanabe & H.Nozaki: "phylogeny of the peculier pigment-containing marine flagellate chlamydomonas parkeae (Chlorophyfa) inferred from the chloroplast ssu rRNA gene sequences" phycologia. 33(予定). (1994)

Y.S Kim、H.Oyaizu、S.Matsumoto、M.M.Watanabe 和 H.Nozaki：“从叶绿体 ssu rRNA 基因序列推断出的含有特殊色素的海洋鞭毛藻 (Chlorophyfa) 的系统发育” phycologia 33（计划）。 ）