Polymorphism of oligosaccharide structure in glycoconjugaates

复合糖中寡糖结构的多态性

基本信息

批准号：
01304030
负责人：
TAKAHASHI Noriko
金额：
$ 10.24万
依托单位：
Nagoya City University College of Nursing
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

项目摘要

Investigators were classified into 3 groups as follows. Investigators belong to groups 1 and 2 were supported with the information obtained by group 3.Group 1 : Analyses of carbohydrate antigens in cancer cells or germ cells and purification of the monoclonal antibodies.Kannagi R. investigated quantitative and qualitative characterization of cancer associated serum glycoprotein antigens expressing sialosyl- or sialosyl-fucosyl type 1 chain polylactosamin.Yamaguchi T. analyzed antigens on human gastric carcinoma.Imai K. investigated a monoclonal antibody to carbohydrate chain on human hepatocellular carcinoma-associated antigen suppressed tumor growth in nude mice.Tsuji Y. investigated human sperm carbohydrate antigens defined by an antisperm human monoclonal antibody derived from an infertile woman bearing antisperm antibodies in her serum.Group 2 : Glycosylation polymorphism on the cell surface, its structure and function.Muramatsu T. and Ozawa M. investigated polymorphism of carbohydrate moieties of peanut agglutinin receptor isolated from human gastric cancer cells.Hayashi K. investigated oligosaccharide polymorphism of nicotinic acetylcholine receptor from Torpedo californica.Nakashima I. investigated cell type and maturation stage-dependent polymorphism of oligosaccharides on murine lymphocytes and lymphoma cells.Kuroda Y. investigated characterization of tumor associated fucogangliosides from PC-12 phenochromocytoma cells.Group 3 : Development of analytical method for simple, reproducible and sensitive determination of the structures of N-linked oligosaccharides.Takahashi N. investigated two-dimensional mapping technique for separation and characterization of N-linked oligosaccharides.Arata Y. accumulated the data of ^1H NMR measurements for N-linked oligosaccharides.Ishihara H. investigated analytical procedure for determination of sialic acids in glycoproteins.

如下，研究人员分为3组。研究人员属于第1组和第2组，并通过第3组获得的信息支持：癌细胞或生殖细胞中碳水化合物抗原的分析，以及单克隆抗体的纯化。 T.分析了人类胃癌的抗原。ImaiK.研究了人类肝细胞癌相关抗原的碳水化合物链的单克隆抗体抑制了裸鼠的肿瘤生长。Tsujiy.Tsuji y.Tsuji y y。从抗原抗原中抑制了抗原抗原抗原抗原，抗原抗原抗原抗原抗原抗原抗原抗原，其血清中的抗体抗体2：细胞表面上的糖基化多态性，其结构和功能。MuramatsuT.和Ozawa M.研究了与人类胃癌的碳水化合物受体分离出的花生果糖素受体的碳水化合物部分的多态性。对人类胃癌的糖果蛋白蛋白蛋白蛋白蛋白酶。来自Torpedo Californica的受体Nakashima I.研究了细胞类型和寡糖对鼠淋巴细胞和淋巴瘤细胞的成熟阶段依赖性二型多态性。KURODAY.研究了肿瘤与PC-12的诱导型细胞的特征。 N-linked oligosaccharides.Takahashi N. investigated two-dimensional mapping technique for separation and characterization of N-linked oligosaccharides.Arata Y. accumulated the data of ^1H NMR measurements for N-linked oligosaccharides.Ishihara H. investigated analytical procedure for determination of sialic acids in glycoproteins.