Analysis of factors relevant to the therapies for immunologically mediated skin diseases.

与免疫介导的皮肤病治疗相关的因素分析。

• 批准号: 63044130
• 负责人: SHIOHARA Tetsuo
• 金额: $ 1.6万
• 依托单位: Kyorin University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Overseas Scientific Survey.
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63044130/
• 关键词: Epidermotropic T-cell clones; Keratinocyte-derived cytokines; Cell adhesion molecules; GM-CSF; IL-6; LFA-1; ICAM-1; Extracorporeal photochemotherapy; GvHD resistance; Thy 1^+ epidermal cell; Fixed drug eruption; ICAM-1; 固定薬疹

1. Mechanism by which certain T cells preferentially migrate into the epidermis was investigated by using murine cloned T-cell lines with or without epidermotropic nature. Expression of GM-CSF receptor was observed on the surface of all epidermotropic T-cell clones tested, although the expression was not restricted to the epidermotropic T-cell clones. LFA-1 was preferentially expressed on the surface of epidermotropic T-cell clones, while non-epidermotropic T-cell clones were negative or very weakly positive for LFA-1 with one exception. Expression of GM-CSF receptor that enables T cells to proliferate in response to keratinocyte-derived GM-CSF and high levels of LFA-1 expression that allows T cells to adhere to epidermis may be a constitutive property of epidermotropic T cells.2. Mice that had spontaneously recovered from the cutaneous GVHD induced by the intradermal injection of epidermotropic T-cell clones became resistant to subsequent attempts to induce the cutaneous GVHD by the T cells. The great increase in the number of Thy 1^+ epidermal cells following destruction of epidermal structures is important in protecting the epidermal integrity from an additional attack by T cells.

1. 通过使用具有或不具有亲表皮性质的小鼠克隆 T 细胞系，研究了某些 T 细胞优先迁移到表皮的机制。在所有测试的亲表皮T细胞克隆的表面上观察到GM-CSF受体的表达，尽管该表达不限于亲表皮T细胞克隆。 LFA-1 优先在亲表皮性 T 细胞克隆表面表达，而非亲表皮性 T 细胞克隆则 LFA-1 呈阴性或极弱阳性，但有一个例外。 GM-CSF受体的表达使T细胞能够响应角质形成细胞来源的GM-CSF而增殖，而LFA-1的高水平表达使T细胞能够粘附到表皮，这可能是亲表皮T细胞的组成特性。2．从皮内注射亲表皮性T细胞克隆诱导的皮肤GVHD中自发恢复的小鼠对随后由T细胞诱导皮肤GVHD的尝试产生了抵抗力。表皮结构破坏后 Thy 1^+ 表皮细胞数量的大幅增加对于保护表皮完整性免受 T 细胞的额外攻击非常重要。

项目成果

Shiohara,T.etal.: "Fixed drug eruption.Expression of epidermal keratinocyte intercellular adhesion molecule-1" Archives of Dermatology. 125. 1371-1376 (1989)

Shiohara，T.etal.：“固定药疹。表皮角质细胞间粘附分子-1 的表达”皮肤病学档案。

Shiohara,T.etal.: "Loss of epidermal integrity by T-cell-mediated attack induces long-term local resistance to subsequent attack.I.Induction of resistance correlates with increases in Thy1^+epidermal cell numbers." Journal of Experimental Medicine. 171. (

Shiohara, T.etal.：“T 细胞介导的攻击导致表皮完整性的丧失会诱导对后续攻击的长期局部抵抗力。I.抵抗力的诱导与 Thy1^ 表皮细胞数量的增加相关。”

Shiohara,T.et al.: "In vitro migration of L3T4^+ cloned T cells to epidermis:Possible role for keratinocyte-derived factors." Journal of Investigative Dermatology. 92. 360-365 (1989)

Shiohara,T.et al.：“L3T4^ 克隆 T 细胞体外迁移至表皮：角质形成细胞衍生因子的可能作用。”

Shiohara, T. et al.: "Loss of epidermal integrity by T-cell-mediated attack induces long-term local resistance to subsequent attack. I. Induction of resistance correlates with increases in Thy 1^+ epidermal cell numbers." Journal of Experimental Medicine.

Shiohara, T. 等人：“T 细胞介导的攻击导致表皮完整性丧失，从而诱导对后续攻击的长期局部抵抗。I. 抵抗力的诱导与 Thy 1^ 表皮细胞数量的增加相关。”

Shiohara, T. et al.: "In vitro migration of L3T4^+ cloned T cells to epidermis: Possible role for keratinocyte-derived factors." Journal of Investigative Dermatology. 92. 360-365 (1989)

Shiohara, T. 等人：“L3T4^ 克隆 T 细胞体外迁移至表皮：角质形成细胞衍生因子的可能作用。”