Investigation of Thomsen-Friedenreich Antigen in Human Bladder Tumors as Detected by Specific Antibody.

通过特异性抗体检测人膀胱肿瘤中 Thomsen-Friedenreich 抗原的研究。

• 批准号: 60570752
• 负责人: FUJITA Kiyoshi
• 金额: $ 1.41万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570752/
• 关键词: T-antigen; 抗T抗体

TUR specimens of non-invasive transitional-cell carcinomas were examined for their expression of Thomsen-Friedenreich (T) antigen by indirect immunofluorescence staining using rabbit IgG antibody which was raised with desialated glycophorin. Nine(45%) out of 20 tumors of low grade (Grade I and II), and 5(56%) of 9 tumors of high grade (III) were diffusely stained with anti-T(T-positive),whereas T-antigen in normal tissue was cryptic and stained only after neuraminidase treatment (cryptic T-positive). Of the T-negative tumors, 9(45%) of the low grade but only one of the high grade tumors, were stained positively for the cryptic T-antigen. The rest of the tumors were devoid of the cryptic T-antigen. Eighty percent of both Tumors expressing T-antigen and those lacking the cryptic T-antigen recurred within two years. Recurrence was not influenced by initial histological grade.

使用用脱唾液酸糖蛋白产生的兔 IgG 抗体进行间接免疫荧光染色，检查非侵袭性移行细胞癌的 TUR 标本中 Thomsen-Friedenreich (T) 抗原的表达。 20 个低级别（I 级和 II 级）肿瘤中的 9 个（45%）和 9 个高级别（III 级）肿瘤中的 5 个（56%）弥漫性抗 T（T 阳性）染色，而 T-正常组织中的抗原是隐性的，仅在神经氨酸酶处理后才染色（隐性 T 阳性）。在 T 阴性肿瘤中，9 个（45%）低级别肿瘤但只有 1 个高级别肿瘤的隐性 T 抗原染色呈阳性。其余的肿瘤缺乏隐性 T 抗原。表达 T 抗原的肿瘤和缺乏隐性 T 抗原的肿瘤中 80% 的肿瘤会在两年内复发。复发不受初始组织学分级的影响。

项目成果

織田英昭,藤田潔,横山雅好,岩田英信,松本充司,吉岡進,竹内正文: 西日本泌尿器科.

Hideaki Oda、Kiyoshi Fujita、Masayoshi Yokoyama、Hienobu Iwata、Mitsushi Matsumoto、Susumu Yoshioka、Masafumi Takeuchi：西日本泌尿外科。

Hideaki Oda, Kiyoshi Fujita, Masayoshi Yokoyama, Hidenobu Iwata, Atsushi Matsumoto, and Masafumi Takeuchi: "Analysis of Adjuvant Therapy for High Stage Bladder Cancer Following Total Cystectomy." Nishinihon Journal of Urology.

Hideaki Oda、Kiyoshi Fujita、Masayoshi Yokoyama、Hienobu Iwata、Atsushi Matsumoto 和 Masafumi Takeuchi：“全膀胱切除术后晚期膀胱癌辅助治疗的分析”。