gas chromatograph coupled with a high-resolution mass spectrometer (GC-MS)

气相色谱仪与高分辨率质谱仪 (GC-MS) 联用

• 批准号: 519403250
• 金额: --
• 依托单位: Universität Leipzig
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/519403250?language=en
• 关键词: gas; chromatograph; coupled; resolution; mass

In chemical ecology, our research focus is on the role of chemical substances that are exchanged between organisms as well as with the environment. We investigate a broad spectrum of questions, ranging from within-species communication (e.g. chemical cues of fertility or kinship) and animal-plant interactions (e.g. seed dispersal by animals, herbivory by insects) to environmental ecology (e.g. effects of pesticides used in monocultures on wildlife and human health). To do so, we investigate the composition of emitted chemicals as well as biological responses to them. Particularly in non-model species, however, significant advances in knowledge are frequently obstructed by the paucity of basal knowledge about the structural properties of the involved chemicals. This bottleneck critically hampers understanding the physiological mechanisms of chemical information transfer as well as its proximate and ultimate functions in intra- and interspecific communication. The present proposal aims to overcome this bottleneck by employing a state-of-the-art gas chromatography-mass spectrometry system (GC-MS), which is optimized for structure elucidation and characterization of unknown substances. The configuration includes a high-resolution MS along with a GC fraction collector for collecting and concentrating target fractions of chemical samples. These fractions allow subjecting target chemicals to a more refined structural analysis or to bioassay tests to investigate the biological responses of various organisms to these fractions. A sniffing port will further allow investigating whether candidate substances and differences between chemical profiles can be perceived by the sensory system of the receiver. Finally, a sample re-collector, that can recapture entire samples, will allow bridging the different components and integrating the requested instrumentation with existing devices to combine the advantages of each instrument for the best-possible performance. Together, this configuration will enable (i) the isolation of biologically active substances from the complex chemical mixtures emitted by plants, animals and their environment, (ii) characterizing the elemental composition of these substances, and (iii) assessing whether these substances are indeed perceivable by the sensory systems of receivers and could thus result in differences at the behavioural level. The system will be used by a team of experienced researchers representing the Faculty of Life Science at Leipzig University, iDiv (German Centre for Integrative Biodiversity Research Halle-Jena-Leipzig), as well as national and international collaborators working on chemical-ecological questions in various taxa at different ecological levels. We anticipate that the requested configuration facilitates major advances in chemical-ecological research, resulting in cooperative and innovative research at an internationally highly competitive level.

在化学生态学中，我们的研究重点是生物体之间以及与环境之间交换的化学物质的作用。我们研究广泛的问题，从物种内交流（例如生育力或亲缘关系的化学线索）和动植物相互作用（例如动物的种子传播、昆虫的食草性）到环境生态学（例如单一栽培中使用的杀虫剂的影响）野生动物和人类健康）。为此，我们研究了排放化学物质的成分以及对它们的生物反应。然而，特别是在非模式物种中，知识的重大进步经常因缺乏有关化学物质结构特性的基础知识而受到阻碍。这一瓶颈严重阻碍了对化学信息传递的生理机制及其在种内和种间交流中的直接和最终功能的理解。本提案旨在通过采用最先进的气相色谱-质谱系统（GC-MS）来克服这一瓶颈，该系统针对未知物质的结构解析和表征进行了优化。该配置包括高分辨率 MS 和 GC 馏分收集器，用于收集和浓缩化学样品的目标馏分。这些组分允许对目标化学品进行更精细的结构分析或生物测定测试，以研究各种生物体对这些组分的生物反应。嗅探端口将进一步研究接收器的传感系统是否可以感知候选物质和化学特征之间的差异。最后，可以重新捕获整个样本的样本重新收集器将允许桥接不同的组件并将所需的仪器与现有设备集成，以结合每种仪器的优点，以获得最佳性能。总之，这种配置将能够（i）从植物、动物及其环境排放的复杂化学混合物中分离生物活性物质，（ii）表征这些物质的元素组成，以及（iii）评估这些物质是否确实是可以被接收者的感觉系统感知，因此可能导致行为层面的差异。该系统将由代表莱比锡大学生命科学学院、iDiv（德国哈雷-耶拿-莱比锡综合生物多样性研究中心）的经验丰富的研究人员团队以及致力于化学生态问题的国内和国际合作者使用。不同生态水平的不同类群。我们预计，所要求的配置将促进化学生态研究的重大进展，从而在国际上具有高度竞争力的水平上进行合作和创新研究。