Anti-Herpes Simplex Virus substances produced by the marine green alga, Dunaliella primolecta

由海洋绿藻 Dunaliella primolecta 产生的抗单纯疱疹病毒物质

• 批准号: 09672290
• 负责人: OHTA Souichi
• 金额: $ 1.86万
• 依托单位: Setsunan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672290/
• 关键词: Anti-Herpes Simplex virus; Dunaliella primolecta; Cytopathic effect; Marine green alga; Dunaliella Primolecta; フェオフィチン; 抗ヘルペスウイルス物質; 植物プランクトン; ドナリエラ; フェオフォルビド; ポルフィリン; CPE抑制

Among 106 microalgae tested, the cytopathic effect (CPE) upon Vero cells of herpes simplex virus, Type 1 (HSV-1) was inhibited by four methanol extracts of Dunaliella bioculata C-523, D.primolecta C-525, Lyngbya sp. M-9 and Lyngbya aerugrneo-coerulea M-12. The green alga, D.primolecta, had the highest anti HSV-1 activity, since 10betagml^<-1> of extract from this alga completely inhibited the CPE.This activity was similar to that of acyclovir at the same concentration. We compared anti-viral activities against adeno virus, herpes simplex virus-2 (HSV-2), Japanese Encephalitis and Polio viruses. Only the CPE of HSV-2 was inhibited. Thus, the factor was specific against HSV.The antiviral activity was apparently excited during HSV adsorption and invasion of the cells. We optimized the conditions for anti HSV- 1 activity by prolonging the exposure of HSV- 1 to the extract. After 2h, the CPE of even a high titer of HSV-1 (10^6 TCID5O/O.1 mL) was completely inactivated. By use of various chromatographic tchniques, three green substances having anti-HSV activity were purified from the algal mass of D.primolecta, and 5 mu g mL^<-1> of this purified substance completely inhibited the CPE.From the analysis of NMR and MS, the chemical structures of the active substances were identified as pheophorbide-like compounds.

在测试的106个微藻中，对单纯疱疹病毒的Vero细胞的细胞病作用（CPE）被d.primolecta c-525，lyngbya sp。 M-9和lyngbya铜绿和lyngbya M-12。绿色藻类d.primolecta具有最高的抗HSV-1活性，因为该藻类的10betagml^<-1>完全抑制了CPE。这种活性与相同浓度下的Acyclovir相似。我们比较了抗病毒活性与Adeno病毒，单纯疱疹病毒-2（HSV-2），日本脑炎和脊髓灰质炎病毒。仅抑制HSV-2的CPE。因此，该因子是针对HSV的特异性因素。在HSV的吸附和细胞浸润过程中，抗病毒活性显然受到激发。我们通过延长HSV-1对提取物的暴露来优化抗HSV-1活性的条件。 2H之后，即使是HSV-1的高滴度（10^6 TCID5O/O.1 mL）的CPE也完全灭活。通过使用各种色谱tchniques，从d.primolecta的藻类质量中纯化了三种具有抗HSV活性的绿色物质，而这种纯化物质的5 mu g ml^<-1>完全抑制了CPE。和MS，活性物质的化学结构被鉴定为嗜phephorbide的化合物。

项目成果

S.Ohta: "Anti-Herpes Somplex Virus substances produced by the marine green alga,Dunaliella primolecta" Journal of Applied Phycology. 10. 349-355 (1998)

S.Ohta：“海洋绿藻，Dunaliella primolecta 产生的抗疱疹病毒物质”应用藻类学杂志。

Souichi Ohta: "Anti-Herpes Simplex Virus substances produced by the marine green alga, Dunaliella primolecta" Journal of Applied Phycology. 10. 349-355 (1998)

Souichi Ohta：“海洋绿藻 Dunaliella primolecta 产生的抗单纯疱疹病毒物质”应用藻类学杂志。

S.Ohta: "Anti-Herpes Simplex Virus substances produced by the marine green alga, Dunaliella Primolecta" Journal of Applied Phycology. 10. 349-355 (1998)

S.Ohta：“海洋绿藻 Dunaliella Primolecta 产生的抗单纯疱疹病毒物质”《应用藻类学杂志》。