DISEASE SPECIFIC ANTIGENS AND T CELLS IN INFLAMMATORY BOWEL DISEASES

炎症性肠病中的疾病特异性抗原和 T 细胞

基本信息

批准号：
09670564
负责人：
MATSUMOTO Takayuki
金额：
$ 1.98万
依托单位：
OSAKA CITY UNIVERSITY MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670564/
关键词：
INFLAMMATORY BOWEL DISEASE CROHN'S DISEASE MUCOSAL IMMUNITY GRANULOMA TH1 CELLS SPECIFIC ANTIGENS 炎症性腸疾患 Th1 クローナリティー T細胞レセプター clonality 腸間膜リンパ節粘膜固有層リンパ球

项目摘要

Inflammatory bowel diseases, including ulcerative colitis and Crohn's disease are chronic refractory inflammatory diseases of intestines with unknown etiology. We have investigated local immune responses featuring both disease, especially with respect to disease specific activation of T cells and disease specific antigenic stimuli by means of the immunohistochemistry and molecular biological techniques.1. T cells or T cell clones generated from involved intestine with Crohn's disease showed oligoclonal expansion in limited variety of T cello receptor BV usages, while those from ulcerative colitis showed polyclonal expansion of T cells.2. Th1 type immune responses were dominant in Crohn's disease, especially around noncaseating granulomas in the intestine and mesenteric lymph nodes. Macrophages and T cells around the granuloma exclusively produced interferon-gamma and interleukin 12 but not interleukin 4. Expanded T cells in Crohn's disease were mainly expressed CD4 and CD45RO suggesting memory T cell expansion.3. These granuloma in Crohn's disease were closely related to the lymphatic system.From these results, Crohn's disease is closely related to the mucosal activation to disease specific antigens.

包括溃疡性结肠炎和克罗恩病在内的炎症性肠道疾病是患有病因未知的肠道的慢性难治性炎症性疾病。我们已经通过免疫组织化学和分子生物学技术调查了既有疾病的局部免疫反应，尤其是在T细胞的特异性激活和特定于疾病的抗原刺激方面的局部免疫反应。1。与克罗恩病有关的肠道产生的T细胞或T细胞克隆在有限的T细胞受体BV使用中显示出寡克隆的膨胀，而溃疡性结肠炎的T细胞膨胀表现出T细胞的多克隆膨胀。2。 Th1型免疫反应在克罗恩病中占主导地位，尤其是在肠道和肠系膜淋巴结中的非造成肉芽肿周围。肉芽肿周围的巨噬细胞和T细胞仅产生干扰素伽马和白介素12而不是白介素4。克罗恩病中的T细胞扩展的T细胞主要表达了CD4，CD45RO表明记忆T细胞扩张。3。克罗恩氏病中的这些肉芽肿与淋巴系统密切相关。从这些结果中，克罗恩病与粘膜激活与疾病特定抗原密切相关。