Steric visualization of diseased organs of laboratory small animals by three-dimensionally reconstructing MRI method

三维重建MRI方法对实验室小动物病变器官的立体可视化

基本信息

批准号：
09556064
负责人：
KUWABARA Mikinori
金额：
$ 8.83万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09556064/
关键词：
MRI three-dimensional steric image laboratory small animal model diseases mouse lung tumor mouse liver tumor volume rendering contrast agent マウス肺腫癌マウス肝腫癌ポリュームレンダリング画像解析小動物疾患三次元画像ラットマウス断層画像非侵襲撮像法

项目摘要

Using multislice MT images of thoracic and abdominal organs in laboratory small animals, three-dimensional (3-d) images were constructed by superimposing them on the computer with the volume ray tracing software. Positive contrast agent was orally administrated into stomach and duodenum cavities just before MR.imaging. Two-dimensional transversal multislices of MR images from thoracic to abdominal regions were taken by an SIS300/183 MRI system with a magnetic field of 7.05 T under the proton-weighting condition (TR=2000ms, TE=20ms). The slice thickness was I mm and the multislice spin-echo method was employed. Each organs (stomach, liver, duodenum, spleen, kidney heart, born and spine) was pseudo-colored by the commercially available graphic software Adobe Photoshop. The 3-d images were then constructed by superimposing these colored-multislices on a UNIX computer by the volume ray tracing method . In rats and mice, the thoracic and abdominal organs were visualized as the pseudo-colored 3-d MR images. The transparent 3-d images were also constructed which made us possible to clearly distinguished individual organ from other organs with respect the position, size and shape. Furthermore, the pathological changes of tissues could be recognized by this 3-d MRI technique in BALB/c mice with liver tumors derived from colon-26 cell line and in A/J mice with lung tumors induced by urethan.

使用实验室小动物胸部和腹部器官的多层 MT 图像，通过体积射线追踪软件将它们叠加在计算机上构建三维 (3-d) 图像。就在 MR 成像之前，将阳性造影剂口服施用到胃和十二指肠腔中。采用SIS300/183 MRI系统在质子加权条件下（TR=2000ms，TE=20ms）在7.05T磁场下拍摄从胸部到腹部的二维横向多切片MR图像。切片厚度为1mm，采用多层自旋回波法。每个器官（胃、肝、十二指肠、脾、肾、心、出生和脊柱）均通过市售图形软件 Adobe Photoshop 进行伪彩色处理。然后通过体积光线追踪方法在 UNIX 计算机上叠加这些彩色多层切片来构建 3D 图像。在大鼠和小鼠中，胸部和腹部器官被可视化为伪彩色 3-d MR 图像。还构建了透明的 3D 图像，使我们能够在位置、大小和形状方面清楚地区分单个器官与其他器官。此外，这种3-d MRI技术可以识别来自结肠26细胞系的肝脏肿瘤的BALB/c小鼠和由尿素诱导的肺部肿瘤的A/J小鼠的组织病理变化。