Immunity system, nerve system ・ endocrine system in periodontitis

牙周炎的免疫系统、神经系统・内分泌系统

• 批准号: 09470427
• 负责人: YOSHINO Hiroshi
• 金额: $ 1.02万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470427/
• 关键词: PERIODONTITIS; NEUROTROPHIN; PERIODONTAL TISSUE; 神経ペプチド; 神経ぺプチド; リヌクファクター

Recently, it has been reported that neurotrophic factors produced non-neural cells which are target cells of peripheral neurons. This study investigated whether nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) and their receptors respectively TRKA, TRKB and TRKC.In a cultured human periodontal ligament cells (HPDL), human gingival fibroblast cells (HGF) and human gingival keratinocytes (HGK), human dental pulp cells (HPC) and human skin fibroblasts (HSF), the mRNA encoding three representative neurotrophins, namely, verve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) and their receptors respectively TRKA, TRKB and TRKC were detected by polymerase chain reaction (PCR) method. All neurotorophins and receptors expressed in almost of cultured cells from periodontal tissue. BDNF in HGK, TRKB in HPDL, NT-3 IN HGK and TRKC in HPC-2 were not demonstrated mRNA almostly.Next, we investigated that the effects of interleukin-1 beta (IL-1) on mRNA demonstration of various neurotrophins and their receptors. TRKA in HPC and NT-3 in HPDL were shown a tendency to increase. But almost of these results might not be significantly. These results may show that neurotrophins do not act in the phase of destroy of periodontal tissue. Namely neurotrophins may promote at the regeneration of periodontal tissue.We also investigated that demonstration of mRNA of various neurotrophins and their receptors in periodontal tissue in vivo by in situ hybridization. Results of in situ hybridization were matched the results of in vitro experiment. Some neurotrophins and their receptors however were showed that various sites in the epidermis. This shows that neurotrophins may act in a paracrine manner.These results show that neurotrophins may act as important factor in the change of periodontal disease state.

最近，据报道，神经营养因子产生的非神经细胞是周围神经元的靶细胞。 This study investigated whether nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) and their receptors respectively TRKA, TRKB and TRKC.In a cultured human periodontal ligament cells (HPDL), human gingival fibroblast cells (HGF) and human gingival keratinocytes (HGK), human dental pulp cells (HPC) and human skin fibroblasts (HSF), the mRNA encoding three representative neurotrophins, namely, verve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) and their receptors respectively TRKA, TRKB and TRKC were detected by polymerase chain reaction （PCR）方法。几乎来自牙周组织的几乎培养细胞中表达的所有神经噬蛋白和受体。 HGK中的BDNF，HPDL中的TRKB，HGK中的NT-3和HPC-2中的TRKC几乎没有证明mRNA。我们调查了白介素-1β（IL-1）对mRNA表现对各种神经营养蛋白及其受体的mRNA的影响。 HPC中的TRKA和HPDL中的NT-3显示出增加的趋势。但是这些结果中几乎可能没有显着。这些结果可能表明，神经营养蛋白不在牙周组织破坏阶段起作用。即神经营养蛋白可能会在牙周组织的再生时促进。我们还研究了通过原位杂交在体内在体内牙周组织中各种神经营养蛋白及其受体的mRNA的表现。原位杂交的结果与体外实验的结果相匹配。然而，一些神经营养蛋白及其受体表明表皮中的各个部位。这表明神经营养蛋白可能以旁分泌作用。这些结果表明，神经营养蛋白可能是牙周疾病状态变化的重要因素。