Investigation of stress response system mediated by HPA-axis in periodontitis

HPA轴介导的牙周炎应激反应系统研究

• 批准号: 15592188
• 负责人: YOSHINO Hiroshi
• 金额: $ 2.18万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592188/
• 关键词: stress; HPA-axis; periodontal tissue; ストレス反応系; HPA axis

Hypothalamic-pituitary-adrenal-axis (HPA-axis) is the main response system for physiological stress. The last productive material of this system is cortisol. It has been reported that cortisol is in saliva and gingival crevicular fluid. And recently, it has been demonstrated that mRNA for HPA-axis components and protein in various peripheral tissues. In this study, we investigated whether there is HPA-axis like system in periodontal tissue or not.(1)mRNA for Corticotropin releasing hormone (CRH), CRH receptor 1 (CRHR1), Proopiomelanocortin (POMC), Prohormone convertase-1 (PC-1), Adrenocorticotropic hormone receptor (ACTHR) and CYP11A1 were demonstrated in culture cells from human periodontal tissue.(2)CRH and ACTH were produced by cultured human gingival fibroblasts and keratinocytes.(3)CRH and ACTH were observed immunohistocemically in periodontal tissue. Positive reaction in tissue from periodontal patients were stronger than from healthy volunteers.These results suggest that there is stress response system at the site of periodontal tissue and this system may work in the periodontitis locally

下丘脑 - 垂体 - 肾上腺轴（HPA轴）是生理压力的主要反应系统。该系统的最后生产材料是皮质醇。据报道，皮质醇在唾液中，牙龈循环液体。最近，已经证明了各种外周组织中HPA轴成分和蛋白质的mRNA。 In this study, we investigated whether there is HPA-axis like system in periodontal tissue or not.(1)mRNA for Corticotropin releasing hormone (CRH), CRH receptor 1 (CRHR1), Proopiomelanocortin (POMC), Prohormone convertase-1 (PC-1), Adrenocorticotropic hormone receptor (ACTHR) and CYP11A1 were demonstrated in （2）CRH和ACTH是由培养的人牙龈成纤维细胞和角质形成细胞产生的。（3）在牙周组织中观察到CRH和ACTH。牙周患者组织中的积极反应比健康的志愿者强。这些结果表明，牙周组织部位有应力反应系统，并且该系统可能在当地牙周炎中起作用