Crosstalk between gibberellin and other signaling molecules in the regulation of seed dormancy

赤霉素与其他信号分子在种子休眠调节中的串扰

• 批准号: RGPIN-2016-05899
• 负责人: Ayele, Belay
• 金额: $ 2.55万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=644351
• 关键词: Crosstalk; between; gibberellin; other; signaling

Gibberellin (GA) is among the plant-produced signaling compounds that influence a wide range of developmental processes at very low concentrations, usually described as “plant hormones”. GA controls the ability of seeds to germinate, or to remain dormant, and this is mediated by the amount of GA present in the seed and seed response to GA. Excess amounts of GA in seeds, or high seed response to the GA, triggers premature germination. Conversely, seeds with low amounts of GA or low response to GA, fail to germinate and remain dormant; and dormancy loss is often associated with increased seed GA level and response. Dormancy control by GA is also mediated by its crosstalk with other signaling molecules that take part in the regulation of seeds' ability to germinate or not, such as jasmonate (JA) and reactive oxygen species (ROS). Seeds employ different strategies to mediate the regulation of GA level and response by other signaling molecules or vice versa, and thereby maintain or lose their state of dormancy. Several of these strategies and the associated regulatory elements have been identified in the seeds of dicots, and understanding this phenomenon has been lagging in monocots, particularly in cereals such as wheat. The overall goal of my research program is to understand how plant hormones regulate developmental processes in sufficient detail to enable genetic manipulation for enhancing crop yield and quality. Within this context, the specific objective of the proposed research is to identify new strategies used by wheat seeds to mediate the control of GA level and response by JA or ROS and vice versa, and thereby control their developmental switch between dormancy and germination. The outcomes of this study not only advance our knowledge of how dormancy is regulated by crosstalk between GA and ROS or JA but also provide new avenues to further increase our understanding of the strategies underlying hormonal control of seed dormancy and germination. The findings of this work will also provide powerful tools for improving the success of seed germination and seedling establishment under diverse environmental conditions and the tolerance of maturing seeds to preharvest sprouting, a serious problem in the production of wheat, thus enhancing yield and quality. Furthermore, this research will train 4 Ph.D., 1 M.Sc. and 5 undergraduate students with a wide range of skills highly valued in the area of plant biology, making the trainees well-positioned to succeed in their career goals while contributing to Canada's need for highly qualified plant biologists.

赤霉素 (GA) 是植物产生的信号化合物之一，可在极低浓度下影响广泛的发育过程，通常被称为“植物激素”，它控制种子发芽或保持休眠的能力。种子中 GA 的含量和种子对 GA 的反应介导。 种子中 GA 含量过高或种子对 GA 的反应较高会引发过早发芽、GA 含量低或对 GA 的反应较低。 GA 无法发芽并保持休眠；并且休眠丧失通常与种子 GA 水平的增加有关，GA 的休眠控制也由其与参与调节种子发芽或能力的其他信号分子的串扰介导。种子采用不同的策略来介导 GA 水平的调节和其他信号分子的反应，反之亦然，从而维持或失去休眠状态。其中一些策略和相关的调控元件已在双子叶植物的种子中被发现，但对单子叶植物的这种现象的理解一直滞后，特别是在小麦等谷物中。我的研究计划的总体目标是了解植物激素如何调节发育。在此背景下，拟议研究的具体目标是确定小麦种子用于介导 JA 或 ROS 等对 GA 水平和反应的控制的新策略。多功能性，从而控制这项研究的结果不仅增进了我们对 GA 和 ROS 或 JA 之间的串扰如何调节休眠的认识，而且还为进一步加深我们对种子休眠的激素控制策略的理解提供了新的途径。这项工作的结果还将为提高不同环境条件下种子发芽和幼苗建立的成功率以及成熟种子对采前发芽的耐受性提供有力的工具，这是生产中的一个严重问题。此外，这项研究还将培养 4 名博士生、1 名硕士生和 5 名本科生，他们在植物生物学领域受到高度重视，使受训者获得良好的发展。 - 定位于成功实现职业目标，同时为加拿大对高素质植物生物学家的需求做出贡献。