Lattice light sheet microscope for fast dual-color long-term imaging

用于快速双色长期成像的点阵光片显微镜

• 批准号: 514074999
• 金额: --
• 依托单位: Technische Universität Dresden
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2023
• 资助国家: 德国
• 起止时间: 2022-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/514074999?language=en
• 关键词: Lattice; light; sheet; microscope; fast

Many important physiological processes such as development and regeneration, biological rhythms, and aging are regulated and manifest at multiple temporal scales. The investigation of these processes requires measurements at multiple levels, ranging from organism and tissue behavior to cellular and molecular events. Hence, to understand complex physiological processes, methodologies are needed that can image these processes with a high temporal and spatial resolution over long time periods. A major challenge in imaging biological processes with high spatial and temporal resolution over extended time periods has been the phototoxicity that is caused by most high-resolution optical sectioning imaging approaches. Light sheet microscopy offers the unique feature of specifically illuminating the currently imaged focal plane, thus resulting in a much gentler illumination of the specimen. Moreover, the advent of the lattice light sheet technology has enabled light sheet imaging at subcellular resolution. A robust lattice light sheet imaging platform allowing for the investigation of light-sensitive biological specimen is essential for many research projects within TU Dresden. Here, we apply for a lattice light sheet platform that will enable fast and gentle multi-color experiments over extended periods of time. The lattice light sheet technology for the first time allows for long-term multicolor imaging of fast biological processes with subcellular resolution. This technology will thus be the basis for the investigation of many highly relevant biological processes. The platform will be set up in the Light Microscopy Facility (LMF) of the Center for Molecular and Cellular Bioengineering (CMCB) at the TU Dresden, thus securing open access and best-possible support for all users.

许多重要的生理过程，例如发育和再生，生物节奏和衰老，都受到多个时间尺度的调节和体现。对这些过程的研究需要在多个水平上进行测量，从生物和组织行为到细胞和分子事件。因此，为了了解复杂的生理过程，需要方法学可以在长期内以高时空和空间分辨率对这些过程进行成像。在延长时期内具有高空间和时间分辨率的生物学过程中的一个主要挑战是光毒性是由大多数高分辨率光学切片成像方法引起的。轻度显微镜提供了专门照亮当前成像的焦平面的独特功能，从而导致样品的柔和照明。此外，晶格轻纸技术的出现使小细胞分辨率的光板成像。一个强大的晶格光片成像平台，允许研究光敏的生物标本，对于Tu Dresden的许多研究项目至关重要。在这里，我们申请了一个晶格轻板平台，该平台将在长时间内实现快速，温和的多色实验。晶格轻纸技术首次允许通过亚细胞分辨率进行快速生物学过程的长期多色成像。因此，该技术将成为研究许多高度相关的生物学过程的基础。该平台将在Tu Dresden的分子和细胞生物工程中心（CMCB）的光学显微镜设施（LMF）中设置，从而确保了所有用户的开放访问和最有可能的支持。