Research of microbe in the deepest subsurface biosphere based on RNA analysis

基于RNA分析的最深层地下生物圈微生物研究

• 批准号: 20810047
• 负责人: HORI Sayaka
• 金额: $ 2.11万
• 依托单位: Japan Agency for Marine-Earth Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (Start-up)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20810047/
• 关键词: 微生物生態系; 有孔虫; 深海; RNA; 極限環境; 微生物; 生態系; 遺伝子発現; バクテリア; 群集・生態系多様性; ゲノム機能・発現; 微生物ゲノム; 遺伝子多様性; 環境徽生物; 分子生物学

To clarify bacterial diversity in the geological static deep-sea sediment, I tried to extract RNA to make cDNA libraries. The Automatic Bottom Inspection and Sampling Mobile "ABISMO" succeeded sampling at the deepest Mariana Trench, however, the included microbe was lower amount than previous report. Then, preliminary experiment was performed using the sediment in the static Izu-Ogasawara Trench. The population of Chloroflexi surrounding giant protist was significantly lower than the plain sediment, suggesting that bacteria in the activity state were distributed heterogeneously in the geologically static environment by coexistence with the large-sized creature.

为了阐明地质静态深海沉积物中的细菌多样性，我尝试提取 RNA 来制作 cDNA 文库。自动海底检查采样移动式“ABISMO”成功在马里亚纳海沟最深处进行了采样，但所含微生物数量比之前的报告要少。然后，利用静态伊豆小笠原海沟的沉积物进行了初步实验。巨型原生生物周围的 Chloroflexi 数量明显低于平原沉积物，表明处于活动状态的细菌通过与大型生物共存而在地质静态环境中分布不均匀。

项目成果

Inactivation of alfa-proteobacteria and activation of gamma-proteobacteria induced by a new candidate genus Xenophyophores (giant foraminifera) at a depth 7111m in the Izu-Ogasawara Trench

伊豆小笠原海沟 7111m 深度的新候选 Xenophyophores 属（巨型有孔虫）诱导的 α-变形菌失活和 γ-变形菌激活

• 发表时间: 2009
• 作者: 堀沙耶香;土屋正史;西真郎;荒井渉;吉田尊雄;高見英人
• 通讯作者: 高見英人

Bacterial diversity surrounding a new candidate genes Xenophyophores (Foraminifera) discovered at a depth 7111m near the Boso Peninsula

在房总半岛附近7111m深度发现的新候选基因Xenophyophores（Foraminifera）周围的细菌多样性

• 发表时间: 2009
• 作者: Sayaka Hori;Tsuchiya Masashi;Shinro Nishi;Wataru Arai;Takao Yoshida;Hideto Takami
• 通讯作者: Hideto Takami