Bio-conversion of Substances and Information by Metal-containing Proteins and Enzymes

含金属蛋白质和酶对物质和信息的生物转化

• 批准号: 16074217
• 负责人: SHIRO Yoshitsugu
• 金额: $ 7.42万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16074217/
• 关键词: Metalloproteins; Bio-synthesis; Signal Transduction; Metal-enzymes; coordination Chemistry; ヘム鉄; 二原子酸素添加酵素; 二成分情報伝達系; センサー蛋白質; 一酸化窒素還元酵素; X線結晶構造解析; 配位構造; ヘム; トリプトファン代謝; キヌレニン経路; 老人性白内障; 感染症; 金属配位空間; ヒスチジンキナーゼ; 酵素センサー蛋白質; エチレンセンサー蛋白質

We have examined conversion of bio-substances and information in molecular level by metal-containing enzymes and proteins such as nitric oxide reductase, indoleamine 2, 3-dioxygenase, and oxygen sensor protein. We proposed the reaction mechansim of conversion of NO (nitric oxide) to N20 (nitrous oxide) on the basis of the ESR results of the reaction intermediates trapped by the rapid mixing-to-quench system, and the crystal structure of this enzyme. We also proposed the reaction mechanism of the tryptophan metabolism catalyzed by indoleamine 2,3-dioxygenase on the basis of its crystal structure. We found that the oxygen sensor protein can descrominate 02 from other two-atomic gas molecules such as CO and NO, and also discussed the pathway of the intra- and inter-molecular signal transduction on the basis of the strcuture of the sensor protein of the thermophilic bacterium, which is analogus to the oxygen sensor protein.

我们已经通过含金属的酶和蛋白质（例如一氧化氮还原酶，吲哚胺2、3-二氧酶和氧传感器蛋白）检查了生物溶剂和分子水平的信息的转化。我们提出了NO（一氧化氮）转化为N20（一氧化二氮）的反应机制，该反应是基于被快速混合到淬灭系统所困扰的反应中间体的ESR结果以及该酶的晶体结构。我们还提出了吲哚胺2,3-二加氧酶催化的色氨酸代谢的反应机理，其晶体结构。我们发现，氧气传感器蛋白可以从其他两种原子气体分子（例如CO和NO）中描述02，并根据嗜热细菌的传感器蛋白的Strcuture，对氧气传感器蛋白的类似物类似，也讨论了分子内和分子间信号转导的途径。

项目成果

Resonance Raman Observation of Structural Dynamics of FixL on Signal Transduction and Ligand Discrimination

FixL 结构动力学对信号转导和配体辨别的共振拉曼观察

• 发表时间: 2007
• 期刊: Biochemistry 46
• 作者: Y. Hiruma;et. al.
• 通讯作者: et. al.

Reaction Mechanism of Heme-containing Oxygenases Based on Their Molecular Structures

基于分子结构的含血红素加氧酶的反应机制

• 发表时间: 2006
• 作者: Shiro Y;Sugimoto H
• 通讯作者: Sugimoto H

酸素添加酵素による物質変換の分子科学

氧化酶物质转化的分子科学

• 发表时间: 2007
• 作者: 城 宜嗣;他3名;城 宜嗣
• 通讯作者: 城 宜嗣

Crystal structure of histidine kinase and response regulator complex: insight into regulatory mechanism of oxygen sensor FixL

组氨酸激酶和反应调节复合物的晶体结构：深入了解氧传感器FixL的调节机制

• 发表时间: 2007
• 作者: Yamada S;Kobayashi M;Sugimoto H;Nakamura H;Shiro Y
• 通讯作者: Shiro Y

Crystallization and Preliminary Crystallographic Studies of Human Indoleamine 2, 3-Dioxygenase

人吲哚胺 2, 3-双加氧酶的结晶和初步晶体学研究

• 发表时间: 2006
• 期刊: Acta Cryst. F62
• 作者: S.Oda;H.Sugimoto;T.Yoshida;Y.Shiro
• 通讯作者: Y.Shiro