Structural elucidation of the sequential signal transduction mechanism in two-component system

双组分系统中顺序信号转导机制的结构阐明

• 批准号: 23790042
• 负责人: UEDA Takumi
• 金额: $ 2.75万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790042/
• 关键词: 構造生物学; NMR; 膜蛋白質; 転移交差飽和法; 過渡的複合体; ニ成分制御系; マルチドメイン蛋白質; 過渡的相互作用; 膜タンパク質; タンパク質―タンパク質相互作用; 交差飽和法; Protein Trans-Splicing; Segmental labeling

Our NMR study based on cross-saturation method revealed that the CheA-CheY complex, which regulates bacterial chemotaxis, exists in equilibrium between catalytic and non-catalytic states, in which their catalytic sites are close to and away from each other, respectively. This binding mode allows CheA to perform two signal transduction reactions with one catalytic site.

我们基于交叉饱和方法的核磁共振研究表明，调节细菌趋化性的CheA-CheY复合物在催化和非催化状态之间存在平衡，其中它们的催化位点分别接近和远离。这种结合模式允许 CheA 通过一个催化位点进行两个信号转导反应。

项目成果

Efficacy of the -2-adrenergic receptor is determined byconformational equilibrium in thetransmembrane region

-2-肾上腺素能受体的功效由跨膜区的构象平衡决定

• 发表时间: 2012
• 期刊: Nature Commun.
• 作者: Yutaka Kofuku;Takumi Ueda;JunyaOkude;Yutaro Shiraishi;Keita Kondo,Masahiro Maeda;Hideki Tsujishita;andIchio Shimada
• 通讯作者: andIchio Shimada

Atypical membrane-embedded p47^<phox> PI(3,4)P_2 binding site on PX domain revealed by NMR

NMR 显示 PX 结构域上非典型膜嵌入 p47^<phox> PI(3,4)P_2 结合位点

• DOI: 10.1074/jbc.m111.332874
• 发表时间: 2012
• 期刊: J. Biol. Chem
• 作者: Stampoulis P;Ueda T;Matsumoto M;Terasawa H;Miyano K;Sumimoto H;and Shimada I
• 通讯作者: and Shimada I

Structural Elucidation of the Alternate Signal Transduction Mechanism of CheA and CheY

CheA 和 CheY 交替信号转导机制的结构阐明

• 发表时间: 2012
• 作者: Takumi Ueda;Masahiko Matsumoto;Ichio Shimada;Yasuhiro Hayashi;Makoto Tsunoda et al.;日比輝正;鳥谷部尚之;Yuichi Minato
• 通讯作者: Yuichi Minato

Functional Equilibrium of the KcsA Structure Revealed by NMR

• DOI: 10.1074/jbc.m112.401265
• 发表时间: 2012-11-16
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Imai, Shunsuke;Osawa, Masanori;Shimada, Ichio
• 通讯作者: Shimada, Ichio

Atypical membrane-embedded phosphatidylinositol3,4-bisphosphate (PI(3,4)P2)-binding site on p47phox Phox homology (PX) domain revealed by NMR.

NMR 显示 p47phox Phox 同源 (PX) 结构域上的非典型膜嵌入磷脂酰肌醇 3,4-二磷酸 (PI(3,4)P2) 结合位点。

• 发表时间: 2012
• 期刊: J. Biol. Chem.
• 作者: Pavlos Stampoulis;Takumi Ueda;Masahiko Matsumoto;Hiroaki Terasawa;and Ichio Shimada
• 通讯作者: and Ichio Shimada