Biospin mediated signaling mechanisms in response to extmcellular stresses

Biospin 介导的响应细胞外应激的信号机制

• 批准号: 15087212
• 负责人: YOSHIMURA Tetsuhiko
• 金额: $ 16.64万
• 依托单位: Yamagata Promotional Organization for Industrial Technology (2004-2006)Yamagata Public Corporation For the Development Of Industry, Institute Life Support Technology (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15087212/
• 关键词: nitric oxide (NO); nitric oxide synthase (NOS); lipopolysaccharide (LPS); Helicobacter pylori; nitrate; gastro-esophageal junction; gastric mucosa; ESR; スピンプローブ法; 植物; ストレス応答機構; オキシデイティブバースト; ジニトロシルジチオラト鉄錯体; in vivo; サクラマス魚卵; ストレス; 酸化還元状態 /

It is now recognized that nitric oxide (NO) is an endogenous mediator of vascular tone, a neurotransmitter in both the peripheral and central nervous systems, and an effector molecule in the immune system. NO is endogenously synthesized from L-arginine by NO synthases (NOSs), which exist in various organs and tissues in mammals. In the gastric mucosa, two types of constitutive NO synthases have been discovered and inducible NO synthase (iNOS), which is expressed in states of inflammation and immune activation, has not been detected in normal rat stomach. Additionally, a non-enzymatic NO production pathway appears to be active in the stomach, in which dietary nitrate is reduced to nitrite by the tongue surface bacteria and the nitrite is then carried into the stomach and reduced to NO under luminal acidic conditions. Endogenous NO, produced through these enzymatic and non-enzymatic pathways, has been demonstrated to be involved not only in the regulation of gastric mucosal blood flow, b … More ut also in the modulation of acid secretion, mucus secretion and gastric motility. In the present study, to elucidate physiological or pathophysiological roles of NO in stomach, we were carried out in vivo study by employing an animal model in which high concentration of NO were generated luminally at the gastro-esophageal junction by administration of nitrite plus acidic ascorbic acid, in vivo study on cross talk between iNOS and cyclooxygenase (COX) in rat gastric mucosa, and in vitro study on the effect of Helicobacter pylori (H. pylori)・lipopolysaccharide (LPS) on the expression of Toll-like receptor (TLR)2 and TLR4 using a mouse normal gastric epithelial cell line (GSM06). Consequently, we obtained following findings : 1) NO generated by nitrite administration on the lumen diffuses into the adjacent gastric tissue to a substantial degree, leading to localized consumption of glutathione in the tissue; 2) the effect of COX activity on iNOS-NO pathway can be important in the regulation of gastric mucosal integrity in inflammation states; 3) H. pylori-LPS stimulation induces TLR2 through TLR4 signaling in normal mouse gastric epithelial GSMO6 cells. This induced TLR2 cooperatively with TLR4 work for iNOS induction by H pylori-LPS simulation. Less

现在人们认识到，一氧化氮 (NO) 是血管张力的内源性介质，是外周和中枢神经系统中的神经递质，也是免疫系统中的效应分子，是由 NO 合酶从 L-精氨酸内源合成的。 NOS）存在于哺乳动物的各种器官和组织中，在胃粘膜中，已发现两种类型的组成型NO合酶和诱导型NO合酶。在正常大鼠胃中未检测到在炎症和免疫激活状态下表达的 iNOS（iNOS）。此外，非酶促 NO 生成途径似乎在胃中活跃，其中饮食中的硝酸盐被还原为亚硝酸盐。然后，舌头表面细菌和亚硝酸盐被携带到胃中，并在腔内酸性条件下还原为一氧化氮。通过这些酶促和非酶促途径产生的内源性一氧化氮已被证明不仅参与胃的调节。粘膜血流量、胃酸分泌、粘液分泌和胃蠕动的调节在本研究中，为了阐明 NO 在胃中的生理或病理生理作用，我们采用动物模型进行了体内研究。通过给予亚硝酸盐和酸性抗坏血酸，在胃食管连接处产生高浓度的 NO，对 iNOS 和 iNOS 之间的串扰进行体内研究大鼠胃粘膜环氧合酶（COX），以及利用小鼠正常胃上皮细胞研究幽门螺杆菌（H. pylori）·脂多糖（LPS）对Toll样受体（TLR）2和TLR4表达影响的体外研究线（GSM06）进行测试，我们得到以下结果：1）亚硝酸盐在管腔上产生的NO扩散到邻近的管腔中。胃组织在很大程度上，导致组织中谷胱甘肽的局部消耗；2) COX 活性对 iNOS-NO 途径的影响对于炎症状态下胃粘膜完整性的调节很重要；刺激通过正常小鼠胃上皮 GSMO6 细胞中的 TLR4 信号传导诱导 TLR2，这诱导 TLR2 与 TLR4 协同作用，通过幽门螺杆菌-LPS 诱导 iNOS。模拟较少。

项目成果

A drastic redox response in plants by environmental stress observed through a novel spin probe

通过新型自旋探针观察到植物因环境胁迫而发生剧烈的氧化还原反应

• 发表时间: 2004
• 期刊: ITE Letters 5(5)
• 作者: T.Nakagawa;T.Yokoyama et al.;M.Aoyama
• 通讯作者: M.Aoyama

EPR imaging to estimate the in vivo intracerebral reducing ability in adolescent rats subjected to neonatal isolation

EPR 成像评估新生大鼠体内脑内还原能力

• 发表时间: 2006
• 期刊: J Magn Reson Imaging (in press)
• 作者: Erabi;K.;Morinobu;s.;et al.;Yokoyama H et al.
• 通讯作者: Yokoyama H et al.

Spinnokinetic analyses of blood disposition and biliary excretion of nitric oxide (NO)-Fe(II)-N-(Dithiocarboxy)sarcosine complex in rats: BCM-ESR and BEM-SER studies

大鼠一氧化氮 (NO)-Fe(II)-N-(二硫羧基)肌氨酸复合物的血液分布和胆汁排泄的自旋动力学分析：BCM-ESR 和 BEM-SER 研究

• 发表时间: 2004
• 期刊: Free Radic Res. 38
• 作者: K.Amemiya;T.Yokoyama et al.;青木直人;H.Yasui
• 通讯作者: H.Yasui

からだの中のラジカルを電子スピン共鳴(ESR)法で見る-ラジカル画像化の方法と最近の進歩

使用电子自旋共振 (ESR) 观察体内自由基 - 自由基成像方法和最新进展

• 发表时间: 2005
• 期刊: 化学と教育 53
• 作者: K. Edamoto;T. Nagayama;K. Ozawa;田中 耕一;J.Yoshitake;J. Yoshitake;K.Uno;N.Ara;K. Uno;N. Ara;K.Asanuma;H.Fujii;H.Morita;N.Kato;S.Kasai;K.Asanuma;D.Suto;吉村哲彦
• 通讯作者: 吉村哲彦

Sequential changes of nitric oxide levels in the temporal lobe on applying nitric oxide synthase inhibitors and phenobarbital in kainic acid-treated mice

使用一氧化氮合酶抑制剂和苯巴比妥对红藻氨酸处理的小鼠颞叶一氧化氮水平的连续变化

• 发表时间: 2005
• 期刊: Epilep.Res. 65
• 作者: K. Edamoto;T. Nagayama;K. Ozawa;田中 耕一;J.Yoshitake;J. Yoshitake;K.Uno;N.Ara;K. Uno;N. Ara;K.Asanuma;H.Fujii;H.Morita;N.Kato
• 通讯作者: N.Kato