NITRIC OXIDE SYNTHASE (ENDOTHELIAL NOS)

一氧化氮合酶（内皮NOS）

• 批准号: 8168598
• 负责人: EDWARD Peter GOGOL
• 金额: $ 0.43万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168598
• 关键词: Blood Pressure; Blood Vessels; Calmodulin; Computer Retrieval of Information on Scientific Projects Database; Cryoelectron Microscopy; Electron Microscope; Enzymes; Funding; Grant; Image; Institution; Nitric Oxide Synthase; Physiological Processes; Research; Research Personnel; Resolution; Resources; Role; Signal Transduction; Source; Structure; United States National Institutes of Health; cell type; constriction; human NOS3 protein; reconstruction

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are examining the structure of nitric oxide synthase (NOS) to understand the conformational changes inherent in its activation by calmodulin (CaM). NOS is the enzyme that generates the intracellular NO signal that influences physiological processes in myraid cell types; biomedically, its role in the constriction of blood vessels is critical in regulating blood pressure. We have imaged and reconstructed the endothelial form of the enzyme (eNOS), in the absence and presence of Ca++/CaM, by cryo-electron microscopy using our local electron microscope facility, a JEOL 1200EXII. The structural resolution of each reconstruction is estimated as 20-25A. we propose to extend the resolution by using the NCMI facility.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 我们正在研究一氧化氮合酶 (NOS) 的结构，以了解钙调蛋白 (CaM) 激活其所固有的构象变化。 NOS 是一种产生细胞内 NO 信号的酶，该信号影响 myraid 细胞类型的生理过程；从生物医学角度来看，其收缩血管的作用对于调节血压至关重要。我们使用我们当地的电子显微镜设备 JEOL 1200EXII 通过冷冻电子显微镜对存在和不存在 Ca++/CaM 的情况下的酶 (eNOS) 的内皮形式进行了成像和重建。每次重建的结构分辨率估计为 20-25A。我们建议使用 NCMI 设施来延长该决议。