Supervision of the Research of Single-cell Molecular Technology

单细胞分子技术研究监督

• 批准号: 11227101
• 负责人: MATSUOKA Hideaki
• 金额: $ 16.77万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11227101/
• 关键词: single-cell; cell function analysis; viability imaging; environmental signal responsive cells; バイアビリティ; 環境シグナル応答; ストレスシグナル応答; イメージング技術; 遺伝子発現制御; 機能評価

This study started in October 1, 1999 and ended in March 31, 2003. In the former half period (1999.10-2001.3), the supervisor group encouraged every investigator to challenge new research subjects focused on the single-cell molecular technology. In the latter half period (2001.4-2003.3), priority was given to several subjects that were regarded as typical single-cell research subjects. They were, for example, "The development of a single-cell experiment system using single-cell manipulation supporting robot" (A-group), "Single-cell bio-imaging using an electrochemical scanning microscope" (B-group), and "The construction of novel plasimids composed of stress response genes and a fluorescent protein reporter gene" (C-group). The expected results of these research subjects were thought to be the important basis of future developmental study on the creation of cells, tissues, and animals with novel functions. In order to discuss about such a prospect, a symposium entitled "From cells to the creation of tissues" was held on November 11-12, 2002. In summary, many experimental know-hows and study results on single-cells were accumulated. Their contribution to biotechnology and bioscience was remarkably great.

本研究从1999年10月1日开始，到2003年3月31日结束。前半期（1999.10-2001.3）导师组鼓励每位研究者挑战以单细胞分子技术为重点的新研究课题。后半期（2001.4-2003.3）优先考虑了几个典型的单细胞研究课题。例如，“使用单细胞操作支持机器人开发单细胞实验系统”（A组）、“使用电化学扫描显微镜的单细胞生物成像”（B组）和“由应激反应基因和荧光蛋白报告基因组成的新型质粒的构建”（C组）。这些研究课题的预期结果被认为是未来创造具有新功能的细胞、组织和动物的发育研究的重要基础。为了探讨这样的前景，2002年11月11日至12日召开了题为“从细胞到组织的创造”的研讨会。 总之，积累了许多单细胞的实验知识和研究成果。他们对生物技术和生物科学的贡献非常巨大。

项目成果

K.Nagai: "RANKL and IL-1 induce the fusion of mononuclear preosteoclasts into multinucleated osteoclasts through their receptors by activating NF-kappaB."Cytotechnology. 33. 203-211 (2000)

K.Nagai：“RANKL 和 IL-1 通过激活 NF-kappaB，通过其受体诱导单核前破骨细胞融合为多核破骨细胞。”细胞技术。

J.Ando: "Sites of Ca^<2+> wave initiation move with caveolae to the trailing edge of migrating cells."J.Cell Sci.. 115. 475-484 (2002)

J.Ando：“Ca ^ 2 > 波起始位点与小凹一起移动到迁移细胞的后缘。”J.Cell Sci.. 115. 475-484 (2002)

T.Matsue: "Oxygen Consumption of Single Bovine Embryos Probed with Scanning Electrochemical Microscopy."Anal.Chem.. 73. 3751-3759 (2001)

T.Matsue：“用扫描电化学显微镜探测单个牛胚胎的耗氧量。”Anal.Chem.. 73. 3751-3759 (2001)

M.Aizawa: "Thermostabiization of protein A-luciferase fusion protein by single amino acid mutation."Biotech.Lett.. 147-149. 24 (2002)

M.Aizawa：“通过单氨基酸突变实现蛋白 A-荧光素酶融合蛋白的热稳定性。”Biotech.Lett.. 147-149。

K.Nagai: "Fenton reaction is primarily involved in a mechanism of (-)-epigallocatechin-3-gallate to induce osteoclastic cell death."Biochem Biophys Res Commun.. 292. 94-101 (2002)

K.Nagai：“芬顿反应主要涉及 (-)-表没食子儿茶素-3-没食子酸酯诱导破骨细胞死亡的机制。”Biochem Biophys Res Commun.. 292. 94-101 (2002)