Development of Immunoanalyzer Based on Gate Function of Protein Membrane

基于蛋白膜门功能的免疫分析仪的研制

• 批准号: 60850141
• 负责人: MATSUOKA Hideaki
• 金额: $ 3.2万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60850141/
• 关键词: Gate functional membrane; Immunoassay; Flavour tracer; Protein membrane; Immunoanalyzer; Human olfactory function; ヒト嗅覚

A novel system of immunoassay has been developed based on the gate function of protein membrane. A monolayer or similar thin layer of protein prepared on the surface of aqueous phase could inhibit the permeation of a flavour compound,versalide.A mixture of antigen (Candida albicans, diameter ca.4 <micro> m) and antibody (immunoglobulin G (IgG)) was reacted at 30゜C for 10 min, and then added on a phosphate buffer solution saturated with versalide. Unreacted IgG remained on the surface of the water. Finally the unreacted IgG covered the whole surface, as monitored by human olfactory function.The amount of added mixture necessary for the coverage was correlated with the concentration of antigen, C.albicans.Antibody bound latex beads were also prepared for the future application to immunoassay for proteins,peptides,and drugs. Immunoanalyzer system was composed of conical reaction cell unit, automatic level controller, and controlling system including a program for operating the whole system.

基于蛋白质膜的门功能，开发了一种新型免疫测定系统，在水相表面制备的单层或类似的薄层蛋白质可以抑制风味化合物Versalide的渗透。抗原混合物（白色念珠菌）。 ，直径约4μm）和抗体（免疫球蛋白G（IgG））在30℃下反应10分钟，然后添加到磷酸盐缓冲溶液中最后，根据人类嗅觉功能的监测，未反应的 IgG 保留在水的表面。覆盖所需的添加混合物的量与抗原白色念珠菌的浓度相关。还制备了抗体结合乳胶珠，以供将来应用于蛋白质、肽和药物的免疫分析。免疫分析仪系统由锥形反应池单元、自动液位控制器和控制系统组成。一个用于操作整个系统的程序。

项目成果

清山哲郎: "バイオセンサ実用便覧" フジテクノシステム, 780 (1986)

清山哲郎：《生物传感器实用手册》Fuji Techno System，780 (1986)

Shoei KATAOKA: Baifukan Co.Sensor Handbook, 1177 (1986)

Shoei KATAOKA：Baifukan Co.传感器手册，1177 (1986)

Analytical Chemistry. 57. 997 (1985)

分析化学。

片岡照栄: "センサハンドブック" 培風館, 1177 (1986)

片冈照卫：《传感器手册》梅风馆，1177 (1986)

Hideaki MATSUOKA: "Pulse Immunoassay for Candida albicans" Anal. Chem.57. 1998-2002 (1985)

Hideaki MATSUOKA：“白色念珠菌的脉冲免疫测定”肛门。