Evaluation of the embryotoxicity of dental alloys containing indium

含铟牙科合金的胚胎毒性评价

基本信息

批准号：
17592055
负责人：
IMAI Koichi
金额：
$ 2.18万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592055/
关键词：
Dental Differentiation Biomaterials Cell ES cell Ag-Pd-Au-Cu-In alloy Ag-In alloy EST EST法溶解度

项目摘要

Dental alloys containing indium, indium alloys with additive ratios of 10%, 15% and 20% ; alloys of silver, palladium, gold, copper and indium with additive ratios of 2%, 4% and 6% ; and a gold alloy for porcelain bonding were examined to evaluate the embryotoxicity risk by accelerated extraction with diamond powder in Dulbecco's Modified Eagle Medium (DMEM). An examination of the embryotoxicity level of each extract using mouse-derived ES-D3 cells demonstrated the possibility of embryotoxicity only for silver-20%indium alloys. The ES-D3 cells in artificial saliva did not differentiate.This suggests elution of the alloy content due to occlusal wear. Accordingly, we simulated the actual conditions of mouth elution of the silver-20% indium alloy, the alloys of silver, palladium, gold, copper and 6%indium using the following procedure. First, anchor teeth for the left and right molars were fixed in a maxillomandibular model, and full cast crowns were made using the above alloys. Finally, the crown surface was polished, and the upper jaw of the model was repeatedly moved for 6 or 12 hours, in order to apply repetitive wear motions to the crown surface. Measurements with ICPS measured the amounts of indium, silver, palladium or copper ions eluted from the alloys to be less than 1 ppm. Furthermore, it was suggested that the embryotoxicity of the alloys of silver, palladium, gold, copper and indium were weaker than those of silver-indium alloys.To examine the supposed embryotoxicity risk of the alloy of Ag-20%, a developmental toxicity test was conducted by the rat whole-embryo culture method. The test results showed that there was no significant difference in yolk-sac diameter, crown-rump length, head length, number of somites and protein content compared to those in the control group. Therefore, no abnormalities were found in the embryos.The results demonstrate that the embryotoxicity risk of alloys containing indium examined in this test is very low.

含铟牙科合金、添加比例为10%、15%、20%的铟合金；添加比例为2%、4%、6%的银、钯、金、铜、铟合金；通过在杜尔贝科改良伊格尔培养基 (DMEM) 中用金刚石粉末加速提取，对陶瓷粘合用金合金进行了检查，以评估胚胎毒性风险。使用小鼠来源的 ES-D3 细胞对每种提取物的胚胎毒性水平进行的检查表明，仅银-20% 铟合金可能具有胚胎毒性。人工唾液中的 ES-D3 细胞没有分化。这表明合金成分由于咬合磨损而洗脱。据此，我们采用以下程序模拟了银-20%铟合金、银、钯、金、铜和6%铟合金的口腔洗脱的实际情况。首先，将左右磨牙的锚固牙固定在上颌模型中，并使用上述合金制作全铸冠。最后，对牙冠表面进行抛光，并重复移动模型的上颌6或12小时，以便对牙冠表面施加重复的磨损运动。使用 ICPS 进行测量，从合金中洗脱的铟、银、钯或铜离子的量低于 1 ppm。此外，还提出银、钯、金、铜、铟合金的胚胎毒性较银铟合金弱。为了检验Ag-20%合金的胚胎毒性风险，进行了发育毒性试验。采用大鼠全胚胎培养法进行。检测结果显示，卵黄囊直径、冠臀长、头长、体节数、蛋白质含量与对照组相比无显着性差异。因此，胚胎中没有发现任何异常。结果表明，本次测试中检测的含铟合金的胚胎毒性风险非常低。