Evaluation of the embryotoxicity of dental alloys containing indium

含铟牙科合金的胚胎毒性评价

基本信息

批准号：
17592055
负责人：
IMAI Koichi
金额：
$ 2.18万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592055/
关键词：
Dental Differentiation Biomaterials Cell ES cell Ag-Pd-Au-Cu-In alloy Ag-In alloy EST EST法溶解度

项目摘要

Dental alloys containing indium, indium alloys with additive ratios of 10%, 15% and 20% ; alloys of silver, palladium, gold, copper and indium with additive ratios of 2%, 4% and 6% ; and a gold alloy for porcelain bonding were examined to evaluate the embryotoxicity risk by accelerated extraction with diamond powder in Dulbecco's Modified Eagle Medium (DMEM). An examination of the embryotoxicity level of each extract using mouse-derived ES-D3 cells demonstrated the possibility of embryotoxicity only for silver-20%indium alloys. The ES-D3 cells in artificial saliva did not differentiate.This suggests elution of the alloy content due to occlusal wear. Accordingly, we simulated the actual conditions of mouth elution of the silver-20% indium alloy, the alloys of silver, palladium, gold, copper and 6%indium using the following procedure. First, anchor teeth for the left and right molars were fixed in a maxillomandibular model, and full cast crowns were made using the above alloys. Finally, the crown surface was polished, and the upper jaw of the model was repeatedly moved for 6 or 12 hours, in order to apply repetitive wear motions to the crown surface. Measurements with ICPS measured the amounts of indium, silver, palladium or copper ions eluted from the alloys to be less than 1 ppm. Furthermore, it was suggested that the embryotoxicity of the alloys of silver, palladium, gold, copper and indium were weaker than those of silver-indium alloys.To examine the supposed embryotoxicity risk of the alloy of Ag-20%, a developmental toxicity test was conducted by the rat whole-embryo culture method. The test results showed that there was no significant difference in yolk-sac diameter, crown-rump length, head length, number of somites and protein content compared to those in the control group. Therefore, no abnormalities were found in the embryos.The results demonstrate that the embryotoxicity risk of alloys containing indium examined in this test is very low.

含有鉴赏剂的牙齿合金，添加剂比为10％，15％和20％；银，钯，黄金，铜和依赖的合金，添加比为2％，4％和6％；并检查了用于瓷粘合的金合金，以通过在Dulbecco改良的Eagle培养基（DMEM）中加速提取钻石粉的提取来评估胚胎毒性风险。使用小鼠衍生的ES-D3细胞对每种提取物的胚胎毒性水平的检查表明，仅对20％的二氮合金具有胚胎毒性。人造唾液中的ES-D3细胞没有区分。这表明由于咬合磨损而洗脱合金含量。因此，我们使用以下程序模拟了银，钯，金，铜和6％indium的银色合金，银合金的嘴巴洗脱条件。首先，左和右磨牙的锚固齿在上颌骨模型中固定，并使用上述合金制成全铸冠。最后，抛光冠表面，并反复移动模型的上颌骨6或12小时，以便在冠表面施加重复的磨损运动。使用ICP的测量测量测量了从合金中洗脱的鉴别，银，钯或铜离子的量小于1 ppm。此外，有人提出，银，钯，金，铜和依赖的金合金的胚胎毒性比银 - 印度合金的蛋白质弱。要检查AG-20％合金的胚胎毒性风险，由Rat-Embryo培养方法进行了发育毒性测试的合金。测试结果表明，与对照组相比，蛋黄 - 囊直径，冠状长度，头部长度，体子数量和蛋白质含量没有显着差异。因此，在胚胎中未发现异常。结果表明，在该测试中检查的合金含有合金的胚胎风险非常低。