In vitro embryotoxicity testing of dental biomaterials by defferentiation of embryonic stem cells

通过胚胎干细胞分化进行牙科生物材料的体外胚胎毒性测试

基本信息

批准号：
12671913
负责人：
IMAI Koichi
金额：
$ 2.43万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671913/
关键词：
differentiation ES cell monomer metal plasticizer tissue model collagen embryotoxicity 歯科材料 ES 分化 ID50 3T3

项目摘要

The effects of dental biomaterials on the differentiation of embryonic stem cells of the mouse cell line D3 (ES-D3cells) were examined using the embryonic stem cell test (EST), which consists of three parameters, differentiation rate into contracting myocard, 50% cell viability of ES-D3 cells and of Balb/c 3T3 cells, and clone A31 (3T3 cells), which were finally calculated by a designated formula. Of the 24 monomers tested, Bis-GMA, UDMA, Bis-MPEPP, TEGDMA, Bis-GMA(6F), 6-HHMA and MTYA were classified as "weak embryotoxic", and 2.0-EPDMA, 3.0-EPDMA, 4.0-EPDMA, 1.6-ADMA, 1.8-ADMA, 1.10-ADMA, HEPC, Phosmer M, BSNa, EDMABA, GAM, GMA, GMR, NPG, PTSNa and QTX as "non embryotoxic". In the case of metal powder extracted in culture media, it was found that Ag was classfied as "weak embryotoxic", while Cot, Cr, Ni and Pd were classified as "non embryotoxic". On the other hand, in the standard chemicals for atomic absorption spectro photometer, it was found that Cr and Hg ions were classified as "strong embryotoxic", and In, Sn, Sb and V ions were classified as "weak embryotoxic", while Ag, Co, Cu, Ni, Pd and Zn ions were classified as "non embryotoxic". Five plasticizers, dibutyl phthalate, n-butyl benzylphthalate, n-Butyl phthalyl n-butyl glycolate, di-2-ethylhexyl phthalate, di-2-ethylhexyladipate were classified as "weak embryotoxic". The effects of hardened dental biomaterials on the differentiation of ES cells in the Type 1 collagen gel matrix were examined. the differentiation rate was decreased in the order of glassionomer cement, two kinds of light cured composite resins, and amalgam, the most severely damaged to almost zero levelIt is considered that further intensive study on the embryotoxicity of dental biomaterials is needed in addition to the conventional biological aspects from the perspective of biological safety

使用胚胎细胞系D3（ES-D3CELLS）的胚胎干细胞分化的牙齿生物材料的影响，使用胚胎干细胞测试（EST）检查，该测试由三个参数组成，分化为肌电肌的分化速率，分化为Myocard，50％的ES-D3细胞的细胞和BALB/C 3T3细胞的细胞生存能力，由BALB/C 3T3细胞和Clone A31（3tt）（3Tt）（3TT）（3TTTCLONE）。 Of the 24 monomers tested, Bis-GMA, UDMA, Bis-MPEPP, TEGDMA, Bis-GMA(6F), 6-HHMA and MTYA were classified as "weak embryotoxic", and 2.0-EPDMA, 3.0-EPDMA, 4.0-EPDMA, 1.6-ADMA, 1.8-ADMA, 1.10-ADMA, HEPC, Phosmer M, BSNa, EDMABA, GAM，GMA，GMR，NPG，PTSNA和QTX为“非胚胎毒性”。对于在培养基中提取的金属粉末，发现Ag被分类为“弱胚胎”，而COT，CR，Ni和PD被分类为“非胚胎毒性”。另一方面，在原子吸收光谱光度计的标准化学物质中，发现Cr和Hg离子被归类为“强胚胎”，而在SN，SB和V离子中被归类为“弱胚胎毒性”，而AG，CO，CU，CU，CU，NI，PD和ZN离子被归类为“非胚胎”。五个增塑剂，邻苯二甲酸二丁酯，N-丁基苯甲酸酯，N-丁基邻苯甲酰基N-丁基乙酸，二-2-乙基苯甲酸苯甲酸苯甲酸酯，DI-2-乙基己基酰基酰基酰胺磷酸酯被分类为“弱胚胎”。研究了硬化牙齿生物材料对1型胶原蛋白凝胶基质中ES细胞分化的影响。差异率降低了玻璃化体水泥的顺序，两种轻质固化的复合树脂和汞合金，最严重的损坏几乎零级别被认为，除了从生物学的角度来看，还需要对牙齿生物材料的胚胎胚胎的进一步深入研究。