In vitro embryotoxicity testing of dental biomaterials by defferentiation of embryonic stem cells

通过胚胎干细胞分化进行牙科生物材料的体外胚胎毒性测试

基本信息

批准号：
12671913
负责人：
IMAI Koichi
金额：
$ 2.43万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671913/
关键词：
differentiation ES cell monomer metal plasticizer tissue model collagen embryotoxicity 歯科材料 ES 分化 ID50 3T3

项目摘要

The effects of dental biomaterials on the differentiation of embryonic stem cells of the mouse cell line D3 (ES-D3cells) were examined using the embryonic stem cell test (EST), which consists of three parameters, differentiation rate into contracting myocard, 50% cell viability of ES-D3 cells and of Balb/c 3T3 cells, and clone A31 (3T3 cells), which were finally calculated by a designated formula. Of the 24 monomers tested, Bis-GMA, UDMA, Bis-MPEPP, TEGDMA, Bis-GMA(6F), 6-HHMA and MTYA were classified as "weak embryotoxic", and 2.0-EPDMA, 3.0-EPDMA, 4.0-EPDMA, 1.6-ADMA, 1.8-ADMA, 1.10-ADMA, HEPC, Phosmer M, BSNa, EDMABA, GAM, GMA, GMR, NPG, PTSNa and QTX as "non embryotoxic". In the case of metal powder extracted in culture media, it was found that Ag was classfied as "weak embryotoxic", while Cot, Cr, Ni and Pd were classified as "non embryotoxic". On the other hand, in the standard chemicals for atomic absorption spectro photometer, it was found that Cr and Hg ions were classified as "strong embryotoxic", and In, Sn, Sb and V ions were classified as "weak embryotoxic", while Ag, Co, Cu, Ni, Pd and Zn ions were classified as "non embryotoxic". Five plasticizers, dibutyl phthalate, n-butyl benzylphthalate, n-Butyl phthalyl n-butyl glycolate, di-2-ethylhexyl phthalate, di-2-ethylhexyladipate were classified as "weak embryotoxic". The effects of hardened dental biomaterials on the differentiation of ES cells in the Type 1 collagen gel matrix were examined. the differentiation rate was decreased in the order of glassionomer cement, two kinds of light cured composite resins, and amalgam, the most severely damaged to almost zero levelIt is considered that further intensive study on the embryotoxicity of dental biomaterials is needed in addition to the conventional biological aspects from the perspective of biological safety

使用胚胎干细胞测试（EST）检查牙科生物材料对小鼠细胞系D3（ES-D3细胞）胚胎干细胞分化的影响，该测试由三个参数组成：收缩心肌的分化率、50％细胞ES-D3细胞和Balb/c 3T3细胞以及克隆A31（3T3细胞）的活力，最终通过指定公式计算。在测试的 24 种单体中，Bis-GMA、UDMA、Bis-MPEPP、TEGDMA、Bis-GMA(6F)、6-HHMA 和 MTYA 被归类为“弱胚胎毒性”，2.0-EPDMA、3.0-EPDMA、4.0-EPDMA , 1.6-ADMA, 1.8-ADMA, 1.10-ADMA, HEPC, Phosmer M, BSNa、EDMABA、GAM、GMA、GMR、NPG、PTSNa 和 QTX 为“无胚胎毒性”。对于从培养基中提取的金属粉末，发现Ag被归类为“弱胚胎毒性”，而Cot、Cr、Ni和Pd被归类为“非胚胎毒性”。另一方面，在原子吸收分光光度计的标准化学品中，发现Cr和Hg离子被归类为“强胚胎毒性”，In、Sn、Sb和V离子被归类为“弱胚胎毒性”，而Ag离子被归类为“弱胚胎毒性”。、Co、Cu、Ni、Pd 和 Zn 离子被归类为“无胚胎毒性”。邻苯二甲酸二丁酯、邻苯二甲酸正丁酯、邻苯二甲酸正丁酯、邻苯二甲酸二-2-乙基己酯、己二酸二-2-乙基己酯等五种增塑剂被列为“弱胚胎毒性”。研究了硬化牙科生物材料对 1 型胶原凝胶基质中 ES 细胞分化的影响。分化率依次降低为玻璃离子水门汀、两种光固化复合树脂、汞合金，损伤最严重，几乎为零水平。认为除常规方法外，还需进一步深入研究牙科生物材料的胚胎毒性。从生物安全角度看生物方面