How does Maitake water extracts inhibit the differentiation of the adipocyte ?

舞茸水提取物如何抑制脂肪细胞的分化？

• 批准号: 17500563
• 负责人: OHTSURU Masaru
• 金额: $ 2.05万
• 依托单位: Mukogawa Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500563/
• 关键词: Maitake; Animal cells; adipocyte; inhibition of differentiation; Functional food

A bioactive substance inhibiting adipocyte differentiation was found in the water-soluble fraction of Maitake (Grifola frondosa). This substance is inhibited triglyceride (TG) accumulation both 3T3-L1, B2C1 and human adipocyte cells.This substance was found to inhibit the differentiation of B_2C_1 preadipocytes, as determined by the decrease in the glycerol-3-phosphate dehydrogenase (GPDH) activity and leptin levels. Furthermore, this substances aren't related to MAP kinase (ERK1 and ERK2) cascade and β-catenin, that inhibited expression of Glut4, peroxisome proliferator activated receptor y (PPARy) and C/EBP α. Moreover, This substances reduced expression time of C/EBP β and C/EBP δ.On the other hand, this substance substance was found to inhibit the differentiation of human preadipocytes, as determined by the decrease in the glycerol-3-phosphate dehydrogenase (GPDH) activity. Furthermore, this factor inhibited the expression of PPARy, C/EBP α, C/EBP β and C/EBP 6, one of the master regulators expressed early in adipocyte differentiation cascades. These results suggest that the application to the person might be able to be expected.The bioactive substance was isolated by HPLC on ODS column, but available water phase. Now, we consider structure of purification sample by LC-MS, GC-MS, NMR.

在舞茸（Grifola frondosa）的水溶性部分中发现了一种抑制脂肪细胞分化的生物活性物质，该物质可以抑制3T3-L1、B2C1和人脂肪细胞的甘油三酯（TG）积累。该物质被发现可以抑制脂肪细胞的分化。 B_2C_1 前脂肪细胞，通过 3-磷酸甘油脱氢酶 (GPDH) 活性和瘦素水平的降低来确定。该物质与 MAP 激酶（ERK1 和 ERK2）级联和 β-连环蛋白无关，可抑制 Glut4、过氧化物酶体增殖物激活受体 y (PPARy) 和 C/EBP α 的表达。此外，该物质还缩短了 C/EBP 的表达时间。 β和C/EBP δ。另一方面，该物质被发现抑制人前脂肪细胞的分化，这通过甘油-3-磷酸的减少来确定。此外，该因子抑制 PPARγ、C/EBP α、C/EBP β 和 C/EBP 6 的表达，这是脂肪细胞分化级联早期表达的主要调节因子之一。生物活性物质是通过 HPLC 在 ODS 柱上分离的，但可用水相。现在，我们通过 LC-MS、GC-MS、NMR 考虑纯化样品的结构。