Analysis of periodontal cell differentiation by gene transfection method

基因转染法分析牙周细胞分化

• 批准号: 16591869
• 负责人: OIDA Shinichiro
• 金额: $ 2.05万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591869/
• 关键词: BMP; TGF-β; Reporter gene; Periodontal ligament cell; siRNA; Retinoic acid; 転写調節因子; 分化誘導; シグナル伝達

Enamel extracts are capable of inducing osteogenesis and cementogenesis. We studied the osteogenetic factors using mouse osteoblastic cell line (ST2, C2C12, MC3T3-E1) and human periodontal ligament (HPDL) cells. The osteoinductive factors examined by specific luciferase reporter gene assay. The results indicated that Enamel extracts contains BMP and TGF-β. BMP induced alkaline phosphatase (ALP) activity and biomineralization, but TGF-β inhibited the induction in mouse osteoblastic cell line. In HPDL cells, TGF-βenhanced alkaline phosphatase (ALP) activity and biomineralization. BMP had no effects on HPDL.BMP and retinoic acid induced ALP synergistically in mouse osteoblastic cells. The signaling pathways of retinoic acid and BMP were examined using RAR subtype( α,β,γ )-specific and samd1-specific siRNAs. RNA γ-specific and smad1-specific siRNA inhibited the ALP induction and biomineralization. The result shows that a RAR γ-dependent functional crosstalk is present between retinoic acid and BMP signaling pathways.Noggin and SB431542 are specific inhibitor of BMP and TGF-β respectively. These specific inhibitors changed the cell differentiation of osteoblastic cell line and HPDL. The result indicates that endogenous BMP and TGF-β are involved in the periodontal cell differentiation. These results suggest that the differentiation factors such as BMP, TGF-β and retinoic acid differentiated the mesenchymal cells in periodontal ligament into periodontal tissue cells.

牙釉质提取物能够诱导成骨和牙骨质形成，我们使用小鼠成骨细胞系（ST2、C2C12、MC3T3-E1）和人牙周膜（HPDL）细胞研究了通过特异性荧光素酶报告基因检测结果检测的成骨因子。表明牙釉质提取物含有 BMP 和 TGF-β 诱导的碱性磷酸酶 (ALP) 活性，并且在 HPDL 细胞中，TGF-β 增强碱性磷酸酶 (ALP) 活性，而 BMP 对 HPDL 没有影响。BMP 和视黄酸在小鼠成骨细胞中协同诱导 ALP。使用 RAR 亚型（α、β、γ）特异性和特异性检查视黄酸和 BMP 的信号通路samd1 特异性 siRNA。RNA γ 特异性和 smad1 特异性 siRNA 抑制 ALP 诱导和生物矿化。结果表明，视黄酸和 BMP 信号通路之间存在 RAR γ 依赖性功能串扰。Noggin 和 SB431542 是 BMP 的特异性抑制剂。这些特异性抑制剂分别改变了成骨细胞系和HPDL的细胞分化。结果表明内源性BMP。这些结果表明，BMP、TGF-β和视黄酸等分化因子使牙周膜间充质细胞分化为牙周组织细胞。

项目成果

Effects of fractionated enamel proteins on human periodontal ligament (HPDL) cells differentiation

分级釉质蛋白对人牙周膜（HPDL）细胞分化的影响

• 发表时间: 2006
• 期刊: Dentistry in Japan 42
• 作者: T.Nagano;et al.
• 通讯作者: et al.

Enamel matrix derivative gel stimulates sigunal transduction of BMP and TGF-β

牙釉质基质衍生凝胶刺激 BMP 和 TGF-β 信号转导

• 发表时间: 2005
• 期刊: Journal of Dental Research 84・6
• 作者: S.Suzuki;T.Nagano;S.Oida et al.
• 通讯作者: S.Oida et al.

Gene expression and localization of insulin-like growth factors and their receptors throughout amelogenesis in rat incisors

• DOI: 10.1369/jhc.5a6821.2005
• 发表时间: 2006-02-01
• 期刊: JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
• 影响因子: 3.2
• 作者: Yamamoto, T;Oida, S;Inage, T
• 通讯作者: Inage, T

Relative levels of mRNA encoding enamel proteins in enamel organ epithelia odontoblasts

釉质器官上皮成牙本质细胞中编码釉质蛋白的 mRNA 相对水平

• 发表时间: 2003
• 期刊: Journal of Dental Research 82・12
• 作者: T.Nagano;S.Oida;H.Ando;K.Gomi;T.Arai;M.Fukae
• 通讯作者: M.Fukae

Porcine enamel protein fractions contain transforming growth factor-β1

• DOI: 10.1902/jop.2006.050352
• 发表时间: 2006-10-01
• 期刊: JOURNAL OF PERIODONTOLOGY
• 影响因子: 4.3
• 作者: Nagano, Takatoshi;Oida, Shinichiro;Fukae, Makoto
• 通讯作者: Fukae, Makoto