Studies on the signal transduction of bone morphogenetic protein (BMP)

骨形态发生蛋白（BMP）信号转导的研究

• 批准号: 06454519
• 负责人: OIDA Shinichiro
• 金额: $ 3.84万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454519/
• 关键词: BMP; receptor; homeodox; ricombinant; baculo-virus; alkaline phosphatase; バキュロウィルス; ホメオボックス遺伝子; 骨誘導

Bone morphogenetic proteins (BMPs), which induce ectopic bone formation in vivo, belong to the transforming growth factor-beta (TGF-beta) superfamily. It is well known that the members play significant and diberse roles in growth and differentiation. The purpose of this study was to investigate the molecular mechanism of bone induction by the BMPs.For the purpose of the application to our studies, we cloned a BMP cDNA from a human dental pulp cell cDNA library and prepared the recombinant BMP using baculovirus/Sf-9 insect cell system. Many experimental systems were examined by this recombinant BMP to determine the useful systems for the study of BMP signal transduction.BMP recputor is the first important molecule for the signal transduction of BMP.After many examination, we cloned a rat BMP receputor cDNA from a cultured dental pulp cells and investigated the expression of the receputor in ectopic bone formation induced by BMP.BMPs locally control bone shape and differentiation in regulating the process of mesenchymal condensation and phenotype expression. In this study, BMP was implanted in various site of the rat and the results indicated that the expression of Msx homeobox genes were detected prior to bone formation. The resut suggests that Msx genes are regulated by BMP and important molecules for ectopic bone formation.

骨形态发生蛋白（BMP）可在体内诱导异位骨形成，属于转化生长因子-β（TGF-β）超家族。众所周知，这些成员在生长和分化中发挥着重要且不同的作用。本研究的目的是探讨BMPs骨诱导的分子机制。为了在我们的研究中应用，我们从人牙髓细胞cDNA文库中克隆了BMP cDNA，并利用杆状病毒/Sf制备了重组BMP。 -9昆虫细胞系统。通过该重组BMP对许多实验系统进行了检验，以确定对BMP信号转导研究有用的系统。BMP受体是BMP信号转导中第一个重要的分子。经过多次检验，我们从培养的大鼠BMP受体cDNA中克隆出来。牙髓细胞并研究了BMP诱导异位骨形成中受体的表达。BMP在调节间充质浓缩和表型表达过程中局部控制骨的形状和分化。在这项研究中，BMP被植入大鼠的不同部位，结果表明Msx同源盒基因的表达在骨形成之前被检测到。结果表明Msx基因受到BMP和异位骨形成的重要分子的调节。

项目成果

M.Goseki, S.Oida et.al.: "Identification of bone-type alkaline phosphatase mRNA from human periodontal ligament cells" J.Dent.Res. 74. 319-322 (1995)

M.Goseki、S.Oida 等人：“人牙周膜细胞中骨型碱性磷酸酶 mRNA 的鉴定”J.Dent.Res。

K.Takeda,S.oida,他: "Expression of bone Morphogenetic Protein genes in the human dental pulp cells" Bone. 15. 467-470 (1994)

K.Takeda、S.oida 等：“人牙髓细胞中骨形态发生蛋白基因的表达”Bone. 15. 467-470 (1994)

S.Kasugai,S.Oida 他: "Expression of prostaglandin E receptor subtypes in bone:Expression of EP_2 in bone development" Bone. 17. 1-4 (1995)

S. Kasugai、S. Oida 等：“骨中前列腺素 E 受体亚型的表达：骨发育中 EP_2 的表达” Bone. 17. 1-4 (1995)

S.Oida,T.Iimura他: "Cloning and sequence of bone morphogenetic protein 4 (BMP-4) from a human placental cDNA library" DNA sequece. 5. 273-275 (1995)

S. Oida、T. Iimura 等人：“来自人胎盘 cDNA 文库的骨形态发生蛋白 4 (BMP-4) 的克隆和测序”DNA 序列。

S.Kasugai,S.Oida: "Expression of prostaglandin E receptor subtype in bone:Expression of EP_2 in bone development" Bone. 17. 1-4 (1995)

S.Kasugai，S.Oida：“骨中前列腺素 E 受体亚型的表达：骨发育中 EP_2 的表达”骨。