The first animal model for "Hit and Run" theory of oncogenesis by EBV related virus

EBV相关病毒致瘤“肇事逃逸”理论的第一个动物模型

• 批准号: 16590314
• 负责人: HAYASHI Kazuhiko
• 金额: $ 2.3万
• 依托单位: National University Corporation Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590314/
• 关键词: EBV; Herpesvirus papio; Rabbit; Hemophagocytic Syndrome; LPD; T-cell

Epstein-Barr virus associated hemophagocytic syndrome (EBV-AHS) is often associated with fatal infectious mononucleosis or T -cell lymphoproliferative diseases (LPD). To elucidate the pathogenesis of fatal Herpesvirus papio (HVP)-infected LPD and subsequently developed HVP-negative lymphomas observed in HVP-induced rabbit hemophagocytic syndrome, we analyzed sequential examination by ultrasonography (US), CT,CBC, antibody titers, viral quantification, liver biopsy of HVP-induced rabbit LPD and lymphoma development. All of the nine Japanese White rabbits inoculated intravenously with HVP died of fatal LPD or lymphomas. LPD was often accompanied by hemophagocytic syndrome (HPS). Sequential laboratory examinations revealed the increase or decrease of anti-EBV-VCA-IgG titers and viral roads. US or CT examination showed the continuously enlarged splenomegaly. Liver biopsy revealed the transient HVP-EBER-1-positive atypical lymphocyte infiltration. Autopsy examination reconfirmed the development of HVP-EBER-1-negative lymphomas in 2 infected rabbits with long survival and HVP-infected LPD in all cases. Hemophagocytic histiocytosis was observed in the lymph nodes, spleen, bone marrow, or thymus. HVP-DNA was detected in the tissues and peripheral blood from the infected rabbits by PCR or Southern blot analysis. These data suggest that HVP-negative lymphomas developed among HVP-positive LPD may be the good model for "hit and run" oncogenesis theory by herpesvirus.

EB 病毒相关噬血细胞综合征 (EBV-AHS) 通常与致命的传染性单核细胞增多症或 T 细胞淋巴细胞增殖性疾病 (LPD) 相关。为了阐明致命性狒狒疱疹病毒 (HVP) 感染的 LPD 以及随后在 HVP 诱导的兔噬血细胞综合征中观察到的 HVP 阴性淋巴瘤的发病机制，我们分析了超声检查 (US)、CT、CBC、抗体滴度、病毒定量、 HVP 诱导的兔 LPD 和淋巴瘤发展的肝活检。静脉注射 HVP 的 9 只日本白兔全部死于致命的 LPD 或淋巴瘤。 LPD常伴有噬血细胞综合征(HPS)。后续实验室检查显示抗 EBV-VCA-IgG 滴度和病毒路径的增加或减少。 US或CT检查显示脾脏持续肿大。肝活检显示短暂的 HVP-EBER-1 阳性非典型淋巴细胞浸润。尸检再次证实 2 只存活时间较长的受感染兔子患有 HVP-EBER-1 阴性淋巴瘤，所有病例均感染 HVP 的 LPD。在淋巴结、脾脏、骨髓或胸腺中观察到噬血细胞组织细胞增多症。通过PCR或Southern印迹分析在感染兔的组织和外周血中检测到HVP-DNA。这些数据表明，HVP 阳性 LPD 中发生的 HVP 阴性淋巴瘤可能是疱疹病毒“肇事逃逸”肿瘤发生理论的良好模型。

项目成果

Expression and intracellular localization of FKHRLI in mammary gland neoplasms

FKHRLI在乳腺肿瘤中的表达和细胞内定位

• 发表时间: 2004
• 期刊: Acta Med.Okayama 58・4
• 作者: Nakamura K.;Senda T.;Sato K.;Mori S.;Moriyama M.;Matano S;Jin Gui-Shan et al.
• 通讯作者: Jin Gui-Shan et al.

Pathobiological characteristics of the child rabbits infected with Epstein-Barr-like virus (Herpesvirus papio) : comparison with pathological features of HVP-infected adult rabbits

感染Epstein-Barr样病毒（狒狒疱疹病毒）的幼兔的病理生物学特征：与感染HVP的成年兔病理特征的比较

• 发表时间: 2006
• 期刊: J Yonago Med Ass 57-3
• 作者: Machide M;Hashigasako A;Matsumoto K;Nakamura T;Ogahara I
• 通讯作者: Ogahara I

Human placenta feeder layers support undifferentiated growth of primate embryonic stem cells

• DOI: 10.1634/stemcells.22-4-433
• 发表时间: 2004-01-01
• 期刊: STEM CELLS
• 影响因子: 5.2
• 作者: Miyamoto, K;Hayashi, K;Ito, Y
• 通讯作者: Ito, Y

Thymic myoid cells as a myasthenogenic antigen and antigen-presenting cells

• DOI: 10.1016/j.jneuroim.2004.01.022
• 发表时间: 2004-05
• 期刊: Journal of Neuroimmunology
• 影响因子: 3.3
• 作者: M. Matsumoto;H. Matsuo;T. Oka;T. Fukudome;K. Hayashi;H. Shiraishi;M. Motomura;N. Shibuya;H. Ayabe

EBV-Associated Diseases in Humans and their Animal In Vivo Models : Part2

人类及其动物体内模型中 EB 病毒相关疾病：第 2 部分

• 发表时间: 2005
• 期刊: Yonago Acta medica 48・2
• 作者: Oshimi K;et al.;Fujimoto M. et al.;Kazuhiko Hayashi
• 通讯作者: Kazuhiko Hayashi