Identification of zooxanthellate genes that are involved in symbiosis with a reef coral, and analyses of their function and expression

鉴定与珊瑚礁共生相关的虫黄藻基因，并分析其功能和表达

基本信息

批准号：
16510157
负责人：
WATANABE Toshiki
金额：
$ 2.43万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16510157/
关键词：
Coral Zooxanthella Symbiosis Gene expression Cloning Bleaching Coral reef

项目摘要

Hermatypic (or reef-building) corals live in obligate mutualistic symbiosis with the symbiotic dinoflagellates Symbiodinium spp. (generally known as zooxanthellae). With the purpose of identifying and characterizing Symbiodinium mRNAs whose expression levels are enhanced in the symbiotic state than in ex hospite conditions, attempts were made to establish a model symbiosis system consisting of newly metamorphosed polyps of the reef coral Acropora tenuis and a monoclonal population of zooxanthellae. To this end, infectivity of several cultured Symbiodinium cell lines was tested on naturally aposymbiotic polyps of A. tenuis. Two clade A strains (PL-TS-1 and CCMP2467) infected the juveniles at high densities, and promoted growth of the host. To identify PL-TS-1 (or A. tenuis) genes involved in the establishment or maintenance of symbiosis, suppression subtractive hybridization was performed. Approx. 50 cDNA clones in the subtracted library were analyzed, but symbiotically induced mRNAs of PL-TS-1 were not represented in the analyzed cDNA population. However, two mRNAs, the expression levels of which were augmented more than twofold by the presence of the symbionts, could be identified. The mRNAs, named AtSym-01 and -02, encode a syndecan family protein with one trans-membrane domain, and an Na^+-independent sulfate transporter of the SLC26A11 family, respectively (it was the first identification on such coral mRNAs). Two actin mRNAs (SyAct-A1 and -A2) in PL-TS-1 were analyzed for the expression levels in the in and ex hospite conditions, and the result strongly suggested that the expression of the SyAct-A2 mRNA was higher in symbiosis with the coral than in in vitro culture.

雌雄同体（或礁石建造）的珊瑚生活在与共生鞭毛菌Symbiodinium spp中的强制性共生中。（通常称为Zooxanthellae）。为了鉴定和表征共生型mRNA，其表达水平在共生状态中而不是在医院条件下增强，试图建立一个模型共生系统，该模型由新近变质的息肉组成，由珊瑚礁珊瑚礁Acropora tenuis和Zooxanthellae的单核种群组成。为此，在A. tenuis的天然无生物息肉上测试了几个培养的共生细胞系的感染性。两个促进菌株（PL-TS-1和CCMP2467）在高密度下感染了少年，并促进了宿主的生长。为了鉴定与建立或维持共生有关的PL-TS-1（或A. tenuis）基因，进行了抑制减法杂交。大约分析了减去的文库中的50个cDNA克隆，但在分析的cDNA种群中未表示PL-TS-1的共生诱导的mRNA。但是，可以鉴定出两个mRNA，即通过共生体的存在增加了两倍以上的表达水平。名为ATSYM-01和-02的mRNA分别编码一个带有一个跨膜结构域的syndecan家族蛋白，分别是SLC26A11家族的Na^+ - 独立的硫酸盐转运蛋白（这是此类珊瑚mrnas的首次识别）。分析了PL-TS-1中的两个肌动蛋白mRNA（SYACT-A1和-A2）的表达水平在IN和EX HOSPITE条件下的表达水平，结果强烈建议SYACT-A2 mRNA的表达与珊瑚的共生性相比，比体外培养更高。

项目成果

期刊论文数量（18）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

海の循環100の危機(渡辺は一部の執筆を担当)

海洋环流100次危机（渡边负责部分撰写）

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
Toshiki Watanabe;ら5名;Ikuko Yuyamaら 7名;Ikuko Yuyama et al. (seven authors);I.Yuyamaら7名;渡辺俊樹;Toshiki Watanabe;渡辺俊樹
通讯作者：
渡辺俊樹

One hundred crises of marine environment (Edited by Ocean Research Institute, The University of Tokyo)

海洋环境的一百个危机（东京大学海洋研究所编）

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
Toshiki Watanabe;ら5名;Ikuko Yuyamaら 7名;Ikuko Yuyama et al. (seven authors);I.Yuyamaら7名;渡辺俊樹;Toshiki Watanabe
通讯作者：
Toshiki Watanabe

Identification of symbiotically expressed coral mRNAs using a model infection system