Basic Research for Small Caliber Regenerative Graft Development Aiming Clinical Application

面向临床应用的小口径再生移植物开发基础研究

• 批准号: 16500286
• 负责人: ISHIDA Atsushi
• 金额: $ 2.37万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500286/
• 关键词: Tissue Engineering; Growth Factor; Artificial Extracellular Matrix; Peripheral Blood Mononuclear Cell; Endothelial Progenitor cell; PTFE; Small Caliber graft; Cell Culture

1.Production of PAU (Polyaminourethane)-coated hydrophilic small caliber regenerative graft and creation of canine model for judging the effectiveness of this new graft : 3mm diameter graft was coated and used for canine cervical artery implantation model, but unfortunately few patent grafts were obtained for evaluation due to intimal hyperplasia of anastomotic site. The patent grafts ware used for evaluation. The PAU-coated graft was histotropic macroscopically and inner-layer of it was covered with less thrombus and endothelial-like cell microscopically.2.Determination of optimal conditions of bone marrow derived endothelial progenitor cell adhesion and proliferation on "PTFE (Polytetrafluoroethylene) sheets" coated with PAU, ECM (extracellular matrix), and growth factors in vitro : G-CSF mobilized peripheral-blood mononuclear cells were used for determination of PAU/ECM combinations on "PTFE sheets" in culture plates. Six ECM, type I & IV Collagen, Fibronectin, 2 Fibronectin peptide and Vitronectin were used for making PAU/ECM combinations. Among PAU/ECM combinations, "type I Collagen+Fibronectin-GRGD peptide+Vitronectin" was most efficient, and "Fibronectin+Vitronectin" was next for G-CSF mobilized peripheral-blood mononuclear cell proliferation on "PTFE sheets".3.Evaluation of the new small caliber regenerative graft coated with PAU/ECM combination based on the data of experiment "2" : It remains to be unclear.

1.生产PAU（聚氨甲烷）涂覆的亲水性小口径再生移植物，并创建犬模型，以判断这种新移植物的有效性：3mm直径的移植物被涂覆并用于犬颈动脉植入模型，但不幸的是，很少有Patent Grafts获得的pate骨移植物。由于吻合部位的内膜增生而引起的评估。专利移植物用于评估。 PAU涂层的移植物是组织宏观宏观的，其内层层被较少的血栓和内皮样细胞显微镜覆盖。2。骨髓衍生的内皮祖细胞粘附的最佳条件的确定性，并在“ poltetraffe（PolytetrafeRafluoRoorooroOtheense）sheers sheerse” ptfe（用PAU，ECM（细胞外基质）和体外的生长因子覆盖：G-CSF动员的周围血液单核细胞用于测定培养板上“ PTFE”板上的PAU/ECM组合。六种ECM，I型和IV胶原蛋白，纤连蛋白，2个纤连蛋白肽和玻璃纤维素用于制造PAU/ECM组合。在PAU/ECM组合中，“ I型胶原蛋白+纤连蛋白-grgd肽+玻璃纤维蛋白”是最有效的，而G-CSF动员的外周核单核细胞在“ PTFE Sheets上”。基于实验“ 2”的数据，涂有PAU/ECM组合的新的小口径再生移植物：尚不清楚。

项目成果

自家末梢血単核球細胞移植血管再生治療、術後持続動注療法併用の下肢血行再建手術

自体外周血单核细胞移植血管再生治疗、下肢血运重建手术联合术后持续动脉灌注治疗

• 发表时间: 2005
• 期刊: 日本心臓血管外科学会雑誌(0285-1474) 34巻臨増
• 作者: 石田 厚;他
• 通讯作者: 他

ハイブリッド型人工血管開発のため、末梢血単核細胞群が生着・増殖に効果的なPTFE人工マトリックスの開発(2)

开发一种可有效促进外周血单核细胞植入和增殖的 PTFE 人工基质，用于开发混合人工血管 (2)

• 发表时间: 2004
• 期刊: 人工臓器(0300-0818) 33巻・2号
• 作者: 石田 厚;他
• 通讯作者: 他

Trial of continuous intra-arterial perfusion therapy after bypass surgery for chronic and acute, poor run-off critical limb ischemia

搭桥手术后连续动脉内灌注治疗慢性和急性、血流量差的严重肢体缺血的试验

• 发表时间: 2005
• 期刊: The Japanese Journal of Vascular Surgery 14-3
• 作者: Ishida;A.;Imamaki;M.;Sakurai;M.;Miyazaki;M.
• 通讯作者: M.

Bypass surgery with autologous peripheral-blood mononuclear cells implantation for foot salvage

自体外周血单核细胞移植搭桥手术保足

• 发表时间: 2005
• 期刊: 日本血管外科学会雑誌 (0918-6778) 14巻・3号
• 作者: Atsushi Ishida;et al.
• 通讯作者: et al.

Is it worthwhile to perform infrapopliteal bypass surgery for non-responders of angiogenesis therapy with autologous peripheral-blood mononuclear cells implantation ?

对于自体外周血单核细胞植入血管生成疗法无反应的患者是否值得进行腘窝下搭桥手术？

• 发表时间: 2004
• 期刊: 日本血管外科学会雑誌(0918-6778) 13巻・2号
• 作者: Atsushi Ishida;et al.
• 通讯作者: et al.