Effects of vasoactive substances on ovarian function and implantation in the molecular biological approach

分子生物学方法探讨血管活性物质对卵巢功能和着床的影响

基本信息

批准号：
14571544
负责人：
KUBOTA Toshiro
金额：
$ 2.18万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571544/
关键词：
nitric oxide (NO)NO synthase (NOS)Carbon monoxide (CO)Heme oxygenase (HO)luteinal function implantation human endometrium granulosa cells Heme oxenase(HO)一酸化炭素妊娠初期絨毛ブタ顆粒膜細胞アポトーシス Fas ligand Heme oxygenase 卵巣機能ブタ卵巣

项目摘要

The aim of the present study was undertaken to investigate some vasoactive substances derived from vascular endotherial cells, namely the nitric oxide(NO)/NO synthase(NOS) system and the carbon monoxide (CO)/heme oxygenase (HO) system, on the differentiation induced by sex steroid hormones in human endometrial cells of the secretory phase and human decidual cells in implantation, and on the follicular development or luteinization in porcine granulose cells by mainly using molecular biological methods. These studies reveal that NO locally synthesized by iNOS could be involved in the control of endometrial functions, and NO/NOS system play an important role in the maintenance of pregnancy. Furthermore, it is suggested that the CO/HO system possibly play a role in the local control of endometrial function in normal menstrual periods. CO/HO may also be involved in the control of placental vascular function, which protect the syncytiotrophoblast and endothelium against oxidative injury. In ovarian functions, these results demonstrate that NO inhibits E2 secretion independent of cGMP by inhibiting P450 aromatase activity in moderately mature PGC, and strongly suggest that the NO/NOS system in PGC regulates steroidogenesis differently during different phase of follicular development. On the other hand, we conclude that products of reactions catalyzed by CO/HO system are likely to be important autocrine/paracrine factors that regulate apoptosis in PGC.

本研究的目的是研究源自血管内皮细胞的一些血管活性物质，即一氧化氮（NO）/NO合酶（NOS）系统和一氧化碳（CO）/血红素加氧酶（HO）系统。主要利用分子生物学手段研究性类固醇激素诱导人分泌期子宫内膜细胞和着床期蜕膜细胞的分化，以及对猪颗粒细胞卵泡发育或黄素化的影响。生物学方法。这些研究表明，iNOS局部合成的NO可能参与子宫内膜功能的控制，NO/NOS系统在妊娠的维持中发挥着重要作用。此外，表明CO/H2O系统可能在正常月经期间子宫内膜功能的局部控制中发挥作用。 CO/H2O还可能参与胎盘血管功能的控制，保护合体滋养层和内皮细胞免受氧化损伤。在卵巢功能中，这些结果表明，NO通过抑制中等成熟PGC中的P450芳香酶活性，独立于cGMP抑制E2分泌，并强烈表明PGC中的NO/NOS系统在卵泡发育的不同阶段以不同方式调节类固醇生成。另一方面，我们得出结论，CO/H2O系统催化的反应产物可能是调节PGC细胞凋亡的重要自分泌/旁分泌因子。