Bone and cartilage regeneration by peripheral blood derived stem cell

通过外周血干细胞进行骨和软骨再生

• 批准号: 14571381
• 负责人: TANAKA Hiroshi
• 金额: $ 2.24万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571381/
• 关键词: peripheral blood derived cell; bone marrow stromal cell; ES cell; osteoblast; chondrocyte; bone formation; cartilage regeneration; gene expression; 抹消血幹細胞; 成長因子; 末梢血管細胞

Cultured cells from peripheral blood or bone marrow were treated with dexamethasone or growth factors. Cells were determined for alkaline phosphatase activity and analyzed by RT PCR for osteoblast markers. Alkaline phosphatase-positive cells were present in bone marrow cells, but not in peripheral blood-derived cells. Gene expression for osteoblast markers were increased in bone marrow cellsDifferentiation for chondrocytePeripheral blood-derived cells, bone marrow cells, fibroblasts, or ES cells were cultured in three-dimensional structure, such as pellet or micromass. Dexamethasone or growth factors were added for three weeks. Histological and immunohistochemical analysis of pellet cultures of bone marrow cells, fibroblasts, and ES cells, but not peripheral blood-derived cells, revealed cartilage-like tissue characterized by metachromatically stained extracellular matrix and type II collagen immunoreactivity, indicative of chondrogenesis

用地塞米松或生长因子处理来自外周血或骨髓的培养细胞。测定细胞的碱性磷酸酶活性，并通过 RT PCR 分析成骨细胞标记物。碱性磷酸酶阳性细胞存在于骨髓细胞中，但不存在于外周血来源的细胞中。骨髓细胞中成骨细胞标志物的基因表达增加软骨细胞的分化将外周血来源的细胞、骨髓细胞、成纤维细胞或ES细胞培养成三维结构，例如颗粒或微团。添加地塞米松或生长因子三周。对骨髓细胞、成纤维细胞和 ES 细胞（但不包括外周血来源细胞）的沉淀培养物进行组织学和免疫组织化学分析，显示软骨样组织的特征是异染染色的细胞外基质和 II 型胶原免疫反应性，表明软骨形成

项目成果

Hiroshi Tanaka 1, Christopher L.Murphy, Chiho Murphy, Mitsuhiro Kimura, Shinya Kawai, Julia M.Polak: "Chondrogenic Differentiation of Murine Embryonic Stem Cells : Effects of Culture Conditions and Dexamethasone"Journal of Cellular Biochemistry. (in press

Hiroshi Tanaka 1、Christopher L.Murphy、Chiho Murphy、Mitsuhiro Kimura、Shinya Kawai、Julia M.Polak：“小鼠胚胎干细胞的软骨分化：培养条件和地塞米松的影响”细胞生物化学杂志。

Tanaka H., Wakisaka A., Ogasa H., Kawai S., Liang C.T.: "Effects of basic fibroblast growth factor on osteoblast-related gene expression in the process of medullary bone formation induced in rat femur."Journal of Bone and Mineral Metabolism. 21. 74-79 (20

Tanaka H.、Wakisaka A.、Ogasa H.、Kawai S.、Liang C.T.：“碱性成纤维细胞生长因子对大鼠股骨髓质骨形成过程中成骨细胞相关基因表达的影响。”骨与矿物质杂志

Hiroshi Tanaka1, Christopher L.Murphy, Chiho Murphy, Mitsuhiro Kimura, Shinya Kawai, Julia M.Polak: "Chondrogenic Differentiation of Murine Embryonic Stem Cells : Effects of Culture Conditions and Dexamethasone"Journal of Cellular Biochemistry. (In press)

Hiroshi Tanaka1、Christopher L.Murphy、Chiho Murphy、Mitsuhiro Kimura、Shinya Kawai、Julia M.Polak：“小鼠胚胎干细胞的软骨分化：培养条件和地塞米松的影响”细胞生物化学杂志。

Tanaka H., Wakisaka A., Ogasa H., Kawai S., Liang C.T.: "Effects of basic fibroblast growth factor on osteoblast-related gene expression in the process of medullary bone formation induced in rat femur"J Bone Miner Metab. 21(2). 74-79 (2003)

Tanaka H.、Wakisaka A.、Ogasa H.、Kawai S.、Liang C.T.：“碱性成纤维细胞生长因子对大鼠股骨髓质骨形成过程中成骨细胞相关基因表达的影响”J Bone Miner Metab。