Effects of Maternal Deprivation on Brain Development in Rats

母亲剥夺对大鼠大脑发育的影响

• 批准号: 14571050
• 负责人: MIKI Takanori
• 金额: $ 2.56万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571050/
• 关键词: maternal deprivation; hippocampal neurons; perinatal stress; brain development; neurotrophic factors; BDNF mRNA; Real-time PCR; stereology; 母性剥奪; ラット海馬; 定量解析; ストレス; 成人病素因; 周生期ストレス; 海馬; 脳発達; 可塑性; 母子間相互作用

We have established the experimental animal (rat) model of maternal deprivation (MD) in human by separating pups from mothers for 3 h/day during postnatal days (PND) 10-15.Effects on body and pituitary weightsThe body weight of MD rats showed no significant difference at PND16, 20 and 30, whereas at PND60 it was high compared to age-matched control. On the other hand, pituitary weight was low at any time points of observation.Effects on brain weightThe brain weight of MD rats showed no significant difference at any time points of observation.Corticosterone concentrationDuring the separation period, blood corticosterone concentration of MD rats was significantly higher (51ng/dL) than that of controls (32ng/dL). After separation, it was declined to 40 ng/dL.Stereological analysis (physical disector)There were no significant difference in total number of pyramidal cells in CA1-3 regions, and granule and hilus neurons in the dentate gyrus of MD rats compared to controls. Additionally, numerical density and the regional volume of MD rats showed no differences compared to controls.Real-time PCR analysis of BDNF mRNAMD rats had less BDNF mRNA in the hippocampus at PND16, whereas at PND 30 and 60 they had much BDNF mRNA than age-matched control animals.

我们通过在出生后10-15天将幼仔与母亲分开3小时/天，建立了人类母性剥夺（MD）的实验动物（大鼠）模型。对体重和垂体重量的影响MD大鼠的体重显示在 PND16、20 和 30 岁时没有显着差异，而在 PND60 时，与年龄匹配的对照组相比，差异较高。另一方面，垂体重量在任何观察时间点均较低。对脑重量的影响MD大鼠的脑重量在任何观察时间点均无显着差异。皮质酮浓度在分离期间，MD大鼠的血皮质酮浓度显着升高。高于对照组 (51ng/dL) (32ng/dL)。分离后下降至40 ng/dL。体视分析（物理解剖）MD大鼠CA1-3区锥体细胞总数以及齿状回颗粒和门神经元数量与对照组相比无显着差异。此外，MD大鼠的数值密度和区域体积与对照组相比没有差异。 BDNF mRNA的实时PCR分析MD大鼠在PND16时海马中BDNF mRNA较少，而在PND 30和60时与年龄相比，MD大鼠海马中BDNF mRNA较多-匹配的对照动物。

项目成果

Identification of a novel variant of the human NR2B gene promoter region and its possible association with schizophrenia

• DOI: 10.1038/sj.mp.4001152
• 发表时间: 2002-01-01
• 期刊: MOLECULAR PSYCHIATRY
• 影响因子: 11
• 作者: Miyatake, R;Furukawa, A;Suwaki, H
• 通讯作者: Suwaki, H

Relationship between cerebral interstitial levels of amino acids and phosphorylation potential during secondary energy failure in hypoxic-ischemic newborn pialets

缺氧缺血新生儿继发性能量衰竭时脑间质氨基酸水平与磷酸化电位的关系

• 发表时间: 2004
• 期刊: Pediatr Res 55
• 作者: Miki T;Satriotomo I;Li H-P;Matsumoto Y;He G;Yokoyam T;Lee K-Y;Bedi KS;Takeuchi Y;Takanori Miki et al.;Takashi Kusaka et al.
• 通讯作者: Takashi Kusaka et al.

Effects of age and alcohol exposure during early life on pyramidal cell numbers in the CA1-CA3 region of the rat hippocampus

• DOI: 10.1002/hipo.10155
• 发表时间: 2004-01-01
• 期刊: HIPPOCAMPUS
• 影响因子: 3.5
• 作者: Miki, T;Harris, SJ;Bedi, KS
• 通讯作者: Bedi, KS

Excessive testosterone treatment and castration induce reactive astrocytes and fos immunoreactivity in suprachiasmatic nucleus of mice.

过量睾酮治疗和去势诱导小鼠视交叉上核中的反应性星形胶质细胞和 fos 免疫反应性。

• 发表时间: 2004
• 期刊: Brain Res 1020
• 作者: Satriotomo et al.

Takashi Kusaka: "Relationship between cerebral interstitial levels of amino acids and phosphorylation potential during secondary energy failure in hypoxic-ischemic newborn piglets"Pediatr Res. 55. 273-279 (2004)

Takashi Kusaka：“缺氧缺血新生仔猪二次能量衰竭期间脑间质氨基酸水平与磷酸化电位之间的关系”Pediatr Res。