Application of Tissue Microarray Method for the Investigation of Gene Expression by Cancer

组织微阵列方法在癌症基因表达研究中的应用

基本信息

批准号：
14570167
负责人：
MUKAI Kiyoshi
金额：
$ 2.05万
依托单位：
Tokyo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Pathological study of cancer starts from morphological observation hollowed by detection of phenotype by immunohistocemistry, gene expression by in situ hybridization and gene analysis by PCR and so on. Paraffin blocks of fonnalin fixed cancer tissue along with their clinical information is an important asset for the pathological studies. Tissue microarray is a method that embed multiple small cores of paraffin-embedded tissue into a new paraffin block Several hundreds of tissue cores maybe put into one paraffin block. This study was aimed to investigate the possible applications of tissue microarray for the systematic studies of cancer. Since this method enables us to study many cases at once, the optimal condition of immunohistochemistry and in situ hybridization was determined by using tissue microarray of many normal tissues fixed for various length of time. It became obvious that the optimal condition for the staining varies depending on the target and the methods/reagents used. The comparison of the results was much easier than using conventional paraffin embedded tissue. Then tissue microarray block containing 271 cases of breast cancer was prepared. Using this block, mRNA of DJ-1, an apotosis related gene was detected by in situ hybridization. At the same time, immunostaining for estrogen/progesteron receptor and Her2 was done using the sections of the same tissue microarray block. The study demonstrated that the expression of DJ-1 was correlated with histological subtype of breast cancer, lymph node metastasis and expression of hormone receptor. From these data, it was suggested that expression of DJ-1 is correlated with malignant potential of breast cancer.Tissue microarray along with the clinical information of the cases is very useful for the systematic study from morphology to phenotype to gene expression and/or gene analysis of various cancers. Further studies will provide important data for the understanding of cancer biology.

癌症的病理学研究从形态学观察开始，通过免疫组化检测表型、通过原位杂交检测基因表达、通过PCR进行基因分析等。福那林固定的癌组织石蜡块及其临床信息是病理研究的重要资产。组织微阵列是一种将石蜡包埋组织的多个小核心嵌入到新的石蜡块中的方法。一个石蜡块中可以放入数百个组织核心。本研究旨在探讨组织微阵列在癌症系统研究中的可能应用。由于这种方法使我们能够同时研究许多病例，因此通过使用固定不同时间长度的许多正常组织的组织微阵列来确定免疫组织化学和原位杂交的最佳条件。很明显，染色的最佳条件根据目标和使用的方法/试剂而变化。结果的比较比使用传统的石蜡包埋组织容易得多。然后制备包含271例乳腺癌的组织微阵列块。使用该块，通过原位杂交检测凋亡相关基因DJ-1的mRNA。同时，使用相同组织微阵列块的切片进行雌激素/孕激素受体和Her2的免疫染色。研究表明DJ-1的表达与乳腺癌的组织学亚型、淋巴结转移及激素受体的表达相关。这些数据表明DJ-1的表达与乳腺癌的恶性潜能相关。组织微阵列以及病例的临床信息对于从形态到表型再到基因表达和/或基因的系统研究非常有用。各种癌症的分析。进一步的研究将为理解癌症生物学提供重要数据。