Study on the biological function of tropical minute rotifers and its application for mass preservation of live food.

热带小轮虫生物学功能及其在活体食品大规模保鲜中的应用研究

基本信息

批准号：
14560155
负责人：
HAGIWARA Atsushi
金额：
$ 2.62万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14560155/
关键词：
tropical minute rotifers genetic characteristics resting egg low temperature tolerance low temperature preservation larval rearing 冷凍保存

项目摘要

1.Genetic characteristics of SS type rotifersCrossbreeding experiments were conducted using three rotifer strains from Indonesia, Thai and Spain strains. Rotifer mating behavior involves male contact on female corona, male circling around female lorica and copulation, followed by fertilization. The frequency of mating was monitored and percent fertilization was obtained, by co-culturing 100 females and 50 males in a 5 ml petri dish. In the crossing between SS strains, percent copulation and fertilization were 5.0-16.6% and 7.7-34.7%, respectively. No copulation and fertilization was observed between S and SS strains in this experiment, whereas such cases were reported with other S and SS strains. A large genetic variation probably exists both in S and SS strain groups.2.Reproduction and mass preservationSS rotifers could be preserved for 14 days at 10 and 12℃. The addition of gamma-aminobutyric acid at 50 ug/mL increased population growth at 25℃. Significantly higher resting egg production was observed when the culture temperature was changed from 30 to 25℃, than when temperature was kept at 30℃. In the large-scale trial using 500L tanks, rotifers produced 2.6x10^6 resting eggs during a 9-day experiment, which was 1.6 times more than the number of rotifers without temperature change.3.Larval rearing of grouper using SS rotifersBy maintaining the SS type rotifer density at 3, 10 and 30 ind./ml, ingestion rate of grouper larvae was reduced on day 6 and 7, and total length of larvae on day 7 was also the smallest. After day 8 however, no difference was found among treatments. The lorica size of supplied SS rotifers ranged from 80-156um (average lorica length=114um), while larvae ingested rotifers whose lorica length was from 75 to 138 um (104 um in average). Thus, grouper larvae at this stage could ingest only smaller size class of SS rotifers.

1.SS型轮虫的遗传特征采用印度尼西亚、泰国和西班牙的3个轮虫品系进行杂交实验，交配行为为雄性接触雌性轮虫、雄性绕雌性轮虫交配，然后进行受精。通过在 5 ml 培养皿中共培养 100 只雌性和 50 只雄性，对 SS 品系之间的杂交进行监测并获得受精百分比。交配和受精率分别为 5.0-16.6% 和 7.7-34.7%。在本实验中，S 和 SS 品系之间未观察到交配和受精情况，而其他 S 和 SS 品系之间可能存在较大的遗传变异。 2.繁殖与大量保存SS轮虫在10℃和12℃下可保存14天。γ-氨基丁酸的添加。在使用 500L 水箱的大规模试验中，当培养温度从 30℃ 变为 25℃ 时，50 ug/mL 的群体生长量显着增加。在为期9天的实验中，轮虫产生了2.6x10^6个休眠卵，是没有温度变化的轮虫数量的1.6倍。3.幼虫使用SS型轮虫饲养石斑鱼通过将SS型轮虫密度维持在3、10和30ind./ml，第6天和第7天石斑鱼幼虫的摄食率降低，第7天幼虫的总长度也最小。然而，第 8 天，各处理之间没有发现差异。所提供的 SS 轮虫的 lorica 大小范围为 80-156um（平均 lorica）。长度=114um），而幼虫摄食的轮虫的长度为75至138um（平均104um），因此，该阶段的石斑鱼幼虫只能摄食较小尺寸的SS轮虫。