BIOLOGICAL ASPECTS OF RESTING EGG DIAPAUSE AND HATCHING AND ITS APPLICATION TO MASS PRESERVATION TECHNOLOGY

静息蛋滞育和孵化的生物学方面及其在大规模保存技术中的应用

• 批准号: 08660235
• 负责人: HAGIWARA Atsushi
• 金额: $ 1.54万
• 依托单位: NAGASAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660235/
• 关键词: ROTIFERA; RESTING EGG; PRESERVATION; DIAPAUSE; HATCHING; CANNING; Brachionus plicatilis; LARVAL REARING; 海産ツボワムシ; 休眠卵; 保存種苗生産

Diapause and hatching of the marine rotifer Brachionus plicatilis Muller resting eggs were examined. The total number of nuclei in an embryo during the internal diapause period increased from 22 on Day 2 post spawn to 39 (each n=1) on Day 6. The outer layr of embryo membrane gradually thickens from 1.2 (Day 0) to 4.0 mum (Day 8) (each n=10). Irradiation at more than 350 nm caused 1-25% hatching, but it reached 50-60% at 250-310nm light. The addition of hydrogen peroxide or prostaglandins (E1, E2 or F2 alpha) caused resting egg hatching even in darkness. The production of peroxide in seawater caused by light as well as the oxidation of fatty acid to prostaglandins inside the embryo is a possible mechanism of resting egg hatching.The hatchability of mass produced resting eggs (stored in sea water under the dark at 4ﾟC) gradually decreased due to bacterial infection of the porous egg surface. The canning method preserves eggs in a dry form, after lyophilization at -40oC.When partial pressures were less than 88 kPa, percent hatching after 6 months ranged between 16 and 20%, similar to that of uncanned eggs (19% hatching), but significantly lower (9 and 11%) at 88 and 101 kpa. Even after 12 months, percent hatching remained at 35% when canning was at 61 kpa. Canned eggs that were treated with NaClO (1ppm) or NFS-Na (5ppm) showed a significantly higher percent hatching (68% and 80%, respectively) than untreated eggs.

在embleil肿瘤周期内核中核中核中核中总核的肿瘤和孵化，从产卵后第2天的22天增加到第6天的第2天（每个n = 1）。从1.2（第0天）到4.0妈妈（第8天）（每个n = 10）。 ，e2或f2 alpha）即使在黑暗中引起的卵也是由光引起的。在4°C下）多孔卵表面的感染。但是，即使在12个月后，在88和101 kPa的情况下显着降低（9％和11％）。 ％和80％）比未生动的鸡蛋。

项目成果

N.Munuswamy et al.: "Structural differences between the resting eggs of Brachionus plicatilis and Brachionus rotundiformis(Rotifera,Brachionidae):an electron microscopic study" Hydrobiologia. 318. 219-223 (1996)

N.Munuswamy 等人：“褶皱臂尾轮虫和圆形臂尾轮虫（轮虫纲，臂轮科）静息卵之间的结构差异：电子显微镜研究”水生生物学。

W.G.Gallardo et al.: "Effect of dimethysulfoxide (dMSO),sodium hydroxide (NaOH),acetone,and ethnol on the population growth,mictic female production,and body size of the rotifer rachionus plicatilis Muller Bull.Fac.Fish.Nagasaki Univ.,No.78(1997)" 長崎大学水産学

W.G.Gallardo 等人：“二甲亚砜 (dMSO)、氢氧化钠 (NaOH)、丙酮和乙醇对褶皱 Rachionus plicatilis 轮虫 Muller Bull.Fac.Fish.Nagasaki Univ 的种群增长、雌性繁殖和体型的影响.,No.78(1997)”长崎大学水产科学

Balompapueng, M.D.et al.: "Preservation of the euryhaline rotifer(Brachionus plicatilis)resting eggs by canning,method." Hydrobiologia. 358. 163-166 (1997)

Balompapueng，医学博士等人：“通过罐装方法保存广盐轮虫（Brachionus plicatilis）休眠卵。”

Balompapueng, M.D., A.Hagiwara, and K.Hirayama: "Preservation of the euryhaline rotifer (Brachionus plicatilis) resting eggs by canning method." Hydrobiologia. 358. 163-166 (1997)

Balompapueng，医学博士、A.Hagiwara 和 K.Hirayama：“通过罐装方法保存广盐轮虫（Brachionus plicatilis）休眠卵。”

Balompapueng. M. D. et al.: "Preservation of the euryhaline rotifer (Brachionus plicatilis) resting eggs by canning method." Hydrobiologia. 358. 163-166 (1998)

巴隆帕蓬。