Studies on postharvest physiology of fruit by using the method for separation of apoplastic and syplastic solution

质外体液分离法研究果实采后生理

基本信息

批准号：
14560018
负责人：
MURAYAMA Hideki
金额：
$ 1.86万
依托单位：
Yamagata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

First, the method for separation of apoplastic and symplastic solutions of fruit flesh was investigated. As a result, it was found to be possible by using a centrifuge.This method applied to apple fruit to clarify the mechanism of watercore development. In‘Fuji' apples, the symptom of watercore was observed 30 days before optimum harvest date, and extended as fruit ripened. Concentrations of sucrose, fructose and glucose in apoplastic solution did not significantly change throughout an experimental period, while sorbitol concentration markedly increased as fruit ripened. In symplastic solution, changes in sugar concentrations were small. In‘Yoko' and‘Orin' apples, watercore did not occurred. In these cultivars, sorbitol concentration in apoplastic solution also increased as fruit ripened, but the levels at optimum harvest date in‘Yoko' and‘Orin' apples were one forth and one fifth of that of‘Fuji' apples, respectively.Secondly, the effect of girdling treatment around the abscission lay … More er of fruit on watercore development and sugar accumulation in‘Fuji' apples was investigated. Consequently, girdling treatment suppressed the occurrence of watercore during ripening. In control fruit, sorbitol concentrations markedly increased especially in apoplastic solution as fruit ripened. In treated fruit, they did not significantly change and were low levels compared with those of control fruit. These results indicated that the accumulation of sorbitol in apoplast was involved in the development of watercore in apple fruit.Finally, the method for separation of apoplastic and symplastic solutions applied to Japanese persimmon fruit to clarify the localization of tannin and the mechanism of deastringency of fruit. Thus, tannin concentration was higher in apoplastic solution than that in symplastic solution. In addition, the bonding of tannin in apoplastic solution and acetaldehyde, which rapidly accumulated after treatment, was thought to be related to rapid destringency by CO_2 treatment in Japanese persimmon fruit. Less

首先，结果是水果新鲜的隔离和符号溶液。在果实中，在果实中延长了果实。果实成熟，但最佳收获日期的水平是'yoko'和'orin'Apples''''fuji'Apple的第五，是敏感的。苹果被调查，尤其是在果实中增加的植物溶液。为了澄清单宁和果实的机理，因此在质塑料溶液中较高的质溶液，在治疗后迅速积累了taldehyde。