Investigation of culturial physiology of tomato by analysis of phytohormone

通过植物激素分析研究番茄的栽培生理

基本信息

批准号：
12660021
负责人：
KOJIMA Kiyohide
金额：
$ 2.37万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660021/
关键词：
tomato phytohormone cytokinins indole-acetic acid apoplast symplast eibberellin abscisic acid IAA ABA LC-MS

项目摘要

As to indole-3-acetic acid(IAA), endogenous levels by liquid chromatography-mass spectrometry, diffusible levels by fluorometric detection and polar transport activity by radioactive IAA were determined in 3-month-old tomato plants. IAA concentration in the apoplast(AP, sap outside a cell) was higher than those in the symplast (SP, sap within a cell) in both upper and lower parts of stems, showing that IAA analysis of AP is important. Younger leaves exported much more diffusible IAA than older leaves. IAA concentration in the main roots was almost at the same level as in the stems. It is suggested that at sampling, the transport capacity of IAA is probably the limiting factor for IAA transport in stems, and the amount of polar IAA transport might be only 19% of the endogenous IAA amount in stems.The levels of IAA, gibberellic acid1 (GAl), trans-zeatin (Z) and trans-zeatin riboside(ZR) in seedless fruits of parthenocarpic tomato were analysed. Stage in 6cm diameter corresponding the middle of cell expansion phase were referred to expansion-stage, and stage in 8cm diameter corresponding the last of cell expansion phase were referred to MG-stage. IAA concentrations of the pericarps and partitions were higher in MG stage than in expansion-stage. In the partitions, IAA concentrations of SP were higher than those of AP in both expansion-and MG-stage. As to Z concentration, the SP of the partitions in expansion-stage had the highest and was 2.7 times than the AP, while in the pericarps were the same level in AP and SP. The ZR concentration in locule tissues in expansion-stage was the highest of all parts. The results suggest that the sites of synthesis may be the SP of partitions for IAA and Z, and locules for ZR.

关于吲哚-3-乙酸（IAA），在3个月大的番茄植物中确定了液相色谱 - 质量光谱法，可通过荧光测定检测的扩散水平和放射性IAA的极性转运活性。凋亡中的IAA浓度（AP，细胞外的SAP）高于茎的上部和下部中的symplast（SP，SAP）中的IAA浓度，表明对AP的IAA分析很重要。年轻的叶子出口的IAA比较老的叶子更具扩散性IAA。主根中的IAA浓度几乎与茎中的水平相同。建议在采样时，IAA的运输能力可能是茎中IAA转运的限制因子，并且极性IAA运输量可能仅为茎中内源性IAA量的19％。分析了（gal），trans-Zeatin（Z）和反式齐丁蛋白（ZR）（ZR）在par苯甲酰番茄的无种子果实中。直径为6厘米的阶段对应于细胞膨胀阶段的中间阶段为膨胀阶段，直径为8厘米的阶段对应于细胞膨胀阶段的最后一个阶段，称为MG阶段。在毫克阶段，果皮和分区的IAA浓度高于扩展阶段。在分区中，在膨胀和MG阶段，IAA的SP浓度高于AP的IAA浓度。至于Z浓度，扩展阶段的分区的SP具有最高，并且比AP的SP高2.7倍，而在Pericarps中，AP和SP的水平相同。膨胀阶段的locule组织中的ZR浓度是所有部分中最高的。结果表明，合成的位点可能是IAA和Z的分区的SP，以及ZR的位置。