Study on differential diagnosis of traumatic or intrinsic cerebral hematoma of the basal nucleus by use of immunohistochemical markers of blood vessels

血管免疫组化标记物鉴别诊断外伤性或内源性基底核血肿的研究

基本信息

批准号：
17590581
负责人：
OGATA Mamoru
金额：
$ 2.24万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

1. Immunohistochemical studies on axonal changes concerning differential diagnosis of intrinsic or traumatic cerebral hematoma : Immunostaining for amyloid precursor protein (APP), neuron-specific enolase (NSE) and neurofilament (NF) 160 labeled varicose axons and axonal bulb in traumatic cases of more than 2 hours-survival. Immunostaining for mitochondria leveled axonal bulb in traumatic cases of more than 1 day-survival. Immunostaining for NF 200 leveled axons regardless of traumatic or non-traumatic cases.2. Immunohistochemical studies on markers of the blood vessels : Immunostaining for angiotensin converting enzyme (ACE) leveled unstable plaques of arteriosclerosis in cases of intrinsic cerebral hematoma. However, ACE labeled plaques in one case of intrinsic hematoma.3. Application to actual autopsy cases : In a case of death 3 weeks after suffering from a traffic accident, an autopsy revealed left extensive cerebral hematoma, cerebral contusion and subarach- noidal hemorrhage. Immunostaining for APP and NSE leveled axonal bulbs. Immunostaining for ACE failed to label vessels. From these findings, we diagnosed that the hematoma is due to trauma. In a case of death after suffering from a lot of skin injuries, an autopsy revealed left extensive cerebral hematoma. Immunostaining for ACE labeled many vessels. From these findings, we judged that the hematoma is due to trauma. In this case, immunostaining for NSE leveled axonal bulbs, suggesting that it should be important to examine several immunostainings before diagnosing.

1。有关轴突变化的免疫组织化学研究，这些轴突变化有关内在或创伤性脑血肿的差异诊断：淀粉样蛋白前体蛋白（APP），神经元特异性烯醇酶（NSE）和神经丝（NF）160标记的杂种轴突和轴突囊泡的免疫染色。在超过1天生存的创伤性病例中，对线粒体升级的轴突灯泡免疫染色。 NF 200升级轴突的免疫染色，无论是创伤或非创伤病例如何。2。关于血管标志物的免疫组织化学研究：血管紧张素转化酶（ACE）在内在的大脑血肿的病例中降低了动脉硬化的不稳定斑块的免疫染色。但是，ACE在一种内在血肿中标记了斑块3。对实际尸检病例的应用：在发生交通事故后3周死亡的情况下，尸检显示剩下的大脑血肿，大脑挫伤和苏巴拉赫 - Noidal出血。用于APP和NSE水平的轴突灯泡的免疫染色。王牌的免疫染色未能标记容器。从这些发现，我们诊断出血肿是由于创伤引起的。在严重皮肤损伤后死亡的情况下，尸检显示剩下的大脑血肿。王牌的免疫染色标记了许多血管。从这些发现中，我们判断出血肿是由于创伤引起的。在这种情况下，对NSE升级的轴突灯泡进行免疫染色，这表明在诊断之前检查几种免疫抑制剂应该很重要。