Studies on morphological changes of the nerve fibers (axon and myelin) after head injury.

头部损伤后神经纤维（轴突和髓磷脂）形态变化的研究。

基本信息

批准号：
06670463
负责人：
OGATA Mamoru
金额：
$ 1.41万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670463/
关键词：
Brain injury Nerve fiber Axon Myelin Forsic pathology Immunohistochemistry Diffuse axonal injury 鍍銀染色

项目摘要

1. Morphological changes of the nerve fibers after head injury : The blocks of the corpus callosum which had been taken in autopsy cases were stained for axon with Holmes' silver method and counter-stained for myelin with luxol fast blue (LFB). Axonal retraction balls (RB) and/or myelin globoids (MG) were detected in sections of patients who survived for more than 2 days after head injury. In addition, all of three axonal changes, i.e.swelling, coarse varicosities and waving, were observed in sections of those who survived for more than 5 hours. These findings were not apparent in sections of control patients.2. Immunohistochemical studies with antibodies to axonal components on damage after head injury : To detect early posttraumatic axonal changes, antibodies to several axonal components such as neuron-specific enolase (NSE), subunits of neurofilament (NF), mitochondria and Alzheimer precursor protein (APP) were employed. Preliminary examination showed that NSE,NF-M and APP could be useful markers for detecting early axonal changes, and that NSE staining found to be most sensitive. Further examination using NSE staining revealed that the antibody to NSE could label RB and swollen axons in sections of patients who survived for only 1 hour after head trauma.3. Immunohistochemical studies with antibodies to myelin components on damage after head injury : To detect posttraumatic myelin changes, antibodies to myelin basic protein and proteolipid protein were employed. MG was identified by both of the myelin immunostaining as well as LFB in sections of patients who survived for more than 2 days. These myelin markers, together with axon markers such as NSE,should be quite useful for estimating the duration of posttraumatic survival.

1。头部受伤后神经纤维的形态变化：用Holmes的银法染色了尸检病例中call体的块，并用luxol Fast Blue（LFB）对髓磷脂进行了染色。在头部受伤后2天以上的患者中检测到轴突回收球（RB）和/或髓磷脂球形（MG）。此外，在存活超过5个小时的人的部分中观察到了三种轴突变化，即旋转，粗静脉曲张和挥舞。这些发现在对照患者的部分中并不明显。2。具有针对轴突成分的抗体损伤后的抗体抗体的免疫组织化学研究：要检测到创伤后轴突变化，对几种轴突成分（如神经元特异性烯醇酶（NSE）），神经丝（NF）的亚基（NF），Mitochonochonochondria和Alzheimer prosise protise protisen（Appsor prosites of App）。初步检查表明，NSE，NF-M和APP可能是检测早期轴突变化的有用标记，并且发现NSE染色最敏感。对NSE染色进行进一步检查表明，NSE的抗体可以在头部创伤后仅存活1小时的患者中标记RB和轴突肿胀。3。对髓磷脂成分的抗体损伤后的免疫组织化学研究：用于检测创伤后髓磷脂的变化，采用了髓磷脂碱性蛋白质和蛋白脂蛋白蛋白的抗体。在生存超过2天的患者中，两种髓磷脂免疫染色和LFB都鉴定出MG。这些髓磷脂标志物以及NSE等轴突标记对于估计创伤后生存的持续时间应该非常有用。