Regulatory mechanisms of production of virulence factor in Vibrio parahaemolyticus

副溶血弧菌毒力因子产生的调控机制

• 批准号: 17590393
• 负责人: TAKAHASHI Akira
• 金额: $ 2.3万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590393/
• 关键词: Vibrio parahaemolyticus; translation; small RNA; post transcriptional regulation; virulence factor

We have examined the effect of NE on the pathogenicity of Vibrio parahaemolyticus. Norepinephrine (NE) is not only a neurotransimitter in the central nervous system, but also is known to modulate the functions of the gut, such as smooth muscle contractibility, submucosal blood flow, and active transepithelial ion transport, through interactions with α-and β-adrenergic receptors. The enterotoxicity of V. parahaemolyticus, assayed by the rat ileal loop model, was increased by NE, and the cell cytotoxicity of V.parahaemolyticus also stimulated by NE.Furthermore, quantity of expression of mRNA of the thermostable direct haemolytsin (TDH) which was main cause of the virulence factor of V. parahemolyticus which participated in diarrhea induction did not change almost, but confirmed that production of TDH increased. And We found that Hfq was important as mRNA combination protein of TDH. This shows that production of TDH is controlled by not only mRNA expression regulation mechanism but also translation regulation mechanism from mRNA to protein.

我们研究了 NE 对副溶血弧菌致病性的影响 去甲肾上腺素 (NE) 不仅是中枢神经系统中的一种神经递质，而且还可以调节肠道功能，例如平滑肌收缩性、粘膜下血流。和活性跨上皮离子转运，通过与 α- 和 β- 肾上腺素能受体相互作用。副溶血弧菌的肠毒性，通过测定。在大鼠回肠袢模型中，NE增加了副溶血弧菌的细胞毒性，并且NE也刺激了副溶血弧菌的细胞毒性。此外，热稳定直接溶血素（TDH）的mRNA表达量是副溶血弧菌毒力因子的主要原因。参与腹泻诱导的副溶血性弧菌几乎没有变化，但证实了TDH的产生增加，并且我们发现Hfq作为TDH的mRNA组合蛋白是重要的。 TDH的产生不仅受到mRNA表达调控机制的控制，而且还受到从mRNA到蛋白质的翻译调控机制的控制。

项目成果

Pore formation of thermostable direct hemolysin secreted from Vibrio parahaemolyticus in lipid bilayers

副溶血弧菌分泌的耐热直接溶血素在脂质双层中的孔形成

• 发表时间: 2006
• 期刊: International Journal of Toxicology 25・5
• 作者: Tomoyasu;T et al.;Isao Watanabe;Osaki Takako;Aosai F.;Kazuhiro Yamamoto;Hiroyuki Yamaguchi;Matsushima R. et al.;Takano Riho;Takahashi A. et al.
• 通讯作者: Takahashi A. et al.

Human airway trypsin-like protease stimulates human bronchial fibroblast proliferation in a protease-activated receptor-2-dependent pathway

• DOI: 10.1152/ajplung.00098.2005
• 发表时间: 2006-02-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
• 影响因子: 4.9
• 作者: Matsushima, R;Takahashi, A;Yasuoka, S
• 通讯作者: Yasuoka, S

Catecholamine-induced stimulation of growth in Vibrio species

• DOI: 10.1111/j.1472-765x.2007.02136.x
• 发表时间: 2007-06-01
• 期刊: LETTERS IN APPLIED MICROBIOLOGY
• 影响因子: 2.4
• 作者: Nakano, M.;Takahashi, A.;Nakaya, Y.
• 通讯作者: Nakaya, Y.

Listeriolysin O-induced membrane permeation mediate persistent IL-6 production in Caco-2 cells during Listeria monocytogenes infection in vitro

李斯特菌溶血素 O 诱导的膜渗透介导单核细胞增生李斯特菌体外感染过程中 Caco-2 细胞持续产生 IL-6

• 发表时间: 2005
• 期刊: Infect. Immum 73・7
• 作者: Kazuhiro;Yamamoto;Atsushi Toyoda;Tsuchiya K.
• 通讯作者: Tsuchiya K.

Pore formation of thermostable direct hemolysin secreted form vibrio parahaemolyticus in lipid bilayers

副溶血弧菌分泌的热稳定性直接溶血素在脂质双层中的孔形成

• 发表时间: 2006
• 期刊: International Journal of Toxicology 25・5
• 作者: Tomoyasu;T et al.;Isao Watanabe;Osaki Takako;Aosai F.;Kazuhiro Yamamoto;Hiroyuki Yamaguchi;Matsushima R. et al.;Takano Riho;Takahashi A. et al.;Norose K.;Kazuhiro Yamamoto;Toyoda Atushi;Matsushima R.et al.;Naoi K.;Isao Watanabe et al.;Takahashi A.et al.;Uruma Tomonori;Kobayashi M.;Kazuhiro Yamamoto et al.;Takahashi A. et al.
• 通讯作者: Takahashi A. et al.