The sensory innervation of the parietal peritoneum in the rat

大鼠壁层腹膜的感觉神经支配

• 批准号: 17590180
• 负责人: TANAKA Koichi
• 金额: $ 0.58万
• 依托单位: Hyogo College of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590180/
• 关键词: Peritoneum; Sensory nerve; Autonomic nervous system; Nerve terminal; Rat; Ultrastructure

The distribution of sensory neurons innervating the parietal peritoneum was studied using axonal transport of fluoro-gold. The tracer was injected to various parts of parietal peritoneum and the diaphragm. After ten days of survival period bilateral dorsal root ganglia from C2 to S6, and the nodose ganglia were dissected. The cryostat sections of these ganglia were mounted on glass slides and observed with a fluorescence microscope. In cases where the tracer was placed on the peritoneum covering the abdominal wall, labeled neurons were observed only in the ipsilateral dorsal root ganglia. A small number of neurons in nodose and cervical dorsal root ganglia of both sides were labeled after placing the tracer on the central part of diaphragm. When fluoro-gold was applied to the peripheral part of the diaphragm, nodose ganglion was negative, and dorsal root ganglia from T6 to T12 were positive. These results suggest that most of the parietal peritoneum receives sensory nerves from dorsal root ganglia.The morphology of these sensory nerve fibers supplying the parietal peritoneum was studied by immunohistochemistry using antisera against neuronal marker, PGP9.5. These axons often formed wavy bundles and ran in various directions. These bundles contained many myelinated and unmyelinated axons. We observed single nerve fibers branching from these bundles, running toward the peritoneal cavity. The single myelinate fibers ran with several Schwann cells, escaped from the myelin sheeth at just beneath the surface, devided into a few processes to reach the peritoneal cavity.

使用氟金的轴突运输研究了支配壁层腹膜的感觉神经元的分布。将示踪剂注射到壁层腹膜和膈肌的各个部位。存活期十天后，解剖双侧背根神经节从C2到S6以及结状神经节。将这些神经节的低温切片安装在载玻片上并用荧光显微镜观察。在将示踪剂放置在覆盖腹壁的腹膜上的情况下，仅在同侧背根神经节中观察到标记的神经元。将示踪剂置于膈肌中央部分后，标记两侧结节和颈部背根神经节的少量神经元。当荧光金涂在膈肌周围部分时，结状神经节呈阴性，T6至T12的背根神经节呈阳性。这些结果表明大部分壁层腹膜接收来自背根神经节的感觉神经。使用针对神经元标记物PGP9.5的抗血清通过免疫组织化学研究了供应壁层腹膜的这些感觉神经纤维的形态。这些轴突通常形成波状束并向各个方向延伸。这些束包含许多有髓鞘和无髓鞘轴突。我们观察到从这些束中分支出单根神经纤维，向腹膜腔延伸。单根髓鞘纤维与数个施万细胞一起运行，从表面正下方的髓鞘层逸出，分成几个过程到达腹膜腔。

项目成果

Expression of aquaporin water channels in the rat pituitary gland

• DOI: 10.1292/jvms.69.1175
• 发表时间: 2007-11-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Kuwahara, Sachi;Maeda, Seisbi;Seki, Makoto
• 通讯作者: Seki, Makoto

Expreseion and localization of aquaporina in the kidney of the Muek Shrew(Suncua Murinus)

Muek Shrew(Suncua Murinus)肾脏中水孔蛋白的表达和定位

• 发表时间: 2008
• 期刊: Journal of Histochemistxy&Cytochemistry 58
• 作者: Seishi Maeda;et. al.
• 通讯作者: et. al.

Morphology and ultrastructure of the sympathetic celiac ganglion neurons projcting to the cardia and pylorus of the rat stomach

大鼠胃贲门和幽门交感腹腔神经节神经元的形态和超微结构

• 发表时间: 2007
• 期刊: Autonomic Neuroscienc : Basic and Clinical 134
• 作者: Tetsu Hayakawa;et. al.
• 通讯作者: et. al.

Morphology and ultrastructure of the sympathetic celiac ganglion neurons projecting to the cardia and pylorus of the rat stomach

投射到大鼠胃贲门和幽门的交感腹腔神经节神经元的形态和超微结构

• 发表时间: 2007
• 期刊: Autonomic Neuroscience:Basic and Clinical 134
• 作者: Tetsu Hayakawa;et. al.
• 通讯作者: et. al.