Analysis of neuropeptide PACAP receptor positive stromal cells in thymus

胸腺神经肽PACAP受体阳性基质细胞分析

• 批准号: 17590164
• 负责人: TOKUDA Nobuko
• 金额: $ 2.24万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590164/
• 关键词: cell ＆ tissue; brain ＆ nerve; radiation; immunology; thymus; neuropeptide; stromal cell; PACAP

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a multifunctional and pleiotropic signal molecule with important activities related to immunity. We previously reported that the PACAP receptor (PAC1-R) is strongly expressed in storomal cells in the medulla of the rat thymus and that its expression in affected by irradiation. In this study we present a detailed investigation of PAC1-R-positive cells in the rat thymus and examine thymus cells of rhesus monkeys TCDD a chemical known to impair thymus and T cells.In the rat thymus, PAC-1-R-positive cells were found in the intermediate type of thymic epithelial cells of the medulla. PAC1-R-potive cells were also seen in the thymic medulla of the rhesus monkey. The thymus showed unusual structures in some rhesus monkey dams and offspring exposed TCDD. Additionally, in these rhesus monkeys, PAC1-R expression was different from that in the control thymus.PACAP has been reported to decrease ischemic neuronal damage and increase IL-6secretion in rats. We also investigated the role played by PACAP and IL-6 in mediating neuroprotection after ischemia. Our results suggest that PACAP prevents neuronal cell death after ischemia via a signaling mechanism involving IL-6.

垂体腺苷酸环化酶激活多肽（PACAP）是一种多功能和多效信号分子，具有与免疫相关的重要活性。我们先前报道说，PACAP受体（PAC1-R）在大鼠胸腺髓质中的仓库细胞中强烈表达，并且其在受照射影响的表达。在这项研究中，我们提出了对大鼠胸腺中PAC1-R阳性细胞的详细研究，并检查了恒河猴的胸腺细胞TCDD TCDD已知可损害胸腺和T细胞的化学物质。在恒河猴的胸腺髓质中也可以看到PAC1-R-potive细胞。胸腺在某些恒河猴大坝和后代暴露的TCDD中显示出异常的结构。此外，在这些恒河猴中，PAC1-R的表达与对照胸腺中的表达不同。据报道，PACAP降低了缺血性神经元损伤并增加大鼠的IL-6分泌。我们还研究了PACAP和IL-6在缺血后介导神经保护方面所扮演的角色。我们的结果表明，PACAP通过涉及IL-6的信号传导机制防止缺血后的神经元细胞死亡。

项目成果

Does PACAP have therapeutic potential in the field of neuroregenerative medicine?

PACAP在神经再生医学领域具有治疗潜力吗？

• 发表时间: 2007
• 作者: Ohtaki H;Nakamachi T;Watanabe J;Yofu S;Matsunaga M;Matsuno R;Dohi K;Shioda S.
• 通讯作者: Shioda S.

Pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1R) colocalizes with activity-dependent neuroprotective protein (ADNP) in the mouse brain.

垂体腺苷酸环化酶激活多肽 (PACAP) 1 型受体 (PAC1R) 与小鼠大脑中的活性依赖性神经保护蛋白 (ADNP) 共定位。

• 发表时间: 2007
• 期刊: Regul Pept (in press)
• 作者: I.Tanii;et al.;Zsolt Lepp;出澤真理;Nakamachi T
• 通讯作者: Nakamachi T

Inhibitory effects of pituitary adenylate cyclase- activating polypeptide (PACAP) and vasoactive intostinal peptide (VIP) on food intake in the goldfish, Carassius auratus.

垂体腺苷酸环化酶激活多肽（PACAP）和血管活性肠肽（VIP）对金鱼摄食的抑制作用。

• 发表时间: 2005
• 期刊: Peptides 26
• 作者: Abdelalim;E.M.;Matsuda K.
• 通讯作者: Matsuda K.

New Frontiers in Regenerative Medicine

• DOI: 10.1007/978-4-431-38208-9
• 发表时间: 2007
• 作者: M. Kusano;S. Shioda

PACAP receptor (PAC1-R) expression in rat and rhesus monkey thymus.

PACAP 受体 (PAC1-R) 在大鼠和恒河猴胸腺中的表达。

• 发表时间: 2006
• 期刊: Ann.N.Y.Acad.Sci. (In press)
• 作者: Ueda;G.;Zabotina T.N.;Blokhin D.Yu.;Sokolovskaya A.A.;Tokuda N.
• 通讯作者: Tokuda N.