Effects of Low-Level Laser Irradiation in Cultured Synovial Cells

低强度激光照射对培养滑膜细胞的影响

• 批准号: 15592070
• 负责人: OHTA Mitsuhiro
• 金额: $ 2.18万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592070/
• 关键词: Laser irradiation; Synovial cell; Cytokine

Immediate effects of low-level laser irradiation (LLLI), such as anti-inflammation and relieving pain against temporomandibular joint(TMJ) disorders, have been reported. However, the molecular based mechanisms are not elucidated yet. Synovial cells are believed to play pathological roles in the development and continuation of inflammation. Cytokine, such as Interleukin(IL)-6,8 and monocyte chemoattractant protein(MCP)-1,has been found in synovial fluid from patients with TMJ synovitis as well as IL-1β.The purpose of this study is examined the effect of LLLI on cytokines production stimulated in human synovial cells treated with IL-1β. The cells were cultured using outgrowth method. The confluent-stage cells were treated with IL-1β as the same time, LLLI was performed to the cells. The amounts of cytokines in conditioned medium were measured by ELISA kit. LLLI significantly reduced the production of IL-6, 8 and MCP-1 in early time. These findings suggest that LLLI may have anti-inflammatory effect on TMJ disorder through the reduction of cytokines production.To determine the effect of prophylactic or therapeutic treatment with low-laser irradiation on paw inflammation, bone and cartilage destruction in type II collagen-induced arthritis(CIA) model in rats. The low-level laser irradiation at prophylactic or therapeutic treatment significantly suppressed the increase in hindpaw swelling and joint destruction. In the prophylactic and therapeutic treatment group at 23 days, swelling inhibition index (%) was respectively 58.1%, 54.4%. The low-level laser irradiation suppressed radiographic changes in hindpaw.These results show that low-level laser irradiation is effective in suppressing inflammation, damage to bone and cartilage in rat CIA, and may increase efficacy of therapy for rheumatoid arthritis.

据报道，诸如抗炎症和临时疼痛（TMJ）的疼痛尚未阐明，基于分子的机制尚未阐明抗炎症和炎症由于白介素（IL）-6,8和单核细胞化学吸引蛋白（MCP）-1，来自TMJ滑膜炎患者以及IL -1β患者的滑动流体以及IL -1β。在使用IL-1β处理的IL-1β中，用IL-1β处理了confluent阶段的细胞这些发现在早期的MCP-1表明，LLLI可能会通过细胞因子的生产来对TMJ疾病产生抗炎作用。 II胶原蛋白诱导的关节炎（CIA）模型在大鼠中。 ％。低水平激光辐照抑制了后爪的IGRAFIC变化。这些结果表明，loan iradiation可有效抑制，对大鼠CIA的损害和软骨的损害增加了治疗的类风湿关节炎的疗效。