Mental stress on saliva chromogranin A in dry mouth

精神压力对口干时唾液嗜铬粒蛋白A的影响

• 批准号: 15592010
• 负责人: IZUMI Masahiro
• 金额: $ 2.37万
• 依托单位: Aichi-gakuin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15592010/
• 关键词: Saliva; Mental stress; Dry mouth; Chromogranin A; Stress marker; 口腔乾燥症

[Background and Purpose] Investigating the exact cause of dry mouth should be important for successful treatment. Among various causes of dry mouth, mental stress is speculated to be a cause of decreasing saliva secretion, although this theory remains under discussion. Recently, saliva chromograninA (CgA) was reported as a new mental stress marker, which facilitated sensitive and non-invasive measurement. In this study, we measured CgA in a limited saliva sample from dry mouth patients. [Methods] We included 40 dry mouth patients (6 males and 34 females, 52-81 years old) and 20 age- and sex-matched healthy subjects. The saliva was simultaneously collected from the bilateral parotid gland and on the surface of oral floor by salivary catheter, and by stype, respectively. The collected saliva was immediately put in the plastic container and frozen. CgA measurement was performed at another specialized institution for single-blind evaluation. We intentionally created some imitation samples consisting of 0.20ml physiologic saline only and mixed these among the saliva samples supplied to the laboratory. [Results] The saliva collected from each region was very limited, ranging from 0.05 to 0.40ml. The CgA measurement of the imitation samples was below 0.05 pmol/ml. There was a good correlation between both parotid glands in CgA, but there was no correlation between the parotid gland and oral floor. The concentration of parotid saliva CgA in healthy subjects was less than 1.02 pmol/ml. There was a marked rise in parotid saliva CgA in 9 dry mouth patients. [Conclusion] The saliva CgA of dry mouth patients could be meaningfully measured even using a limited sample. Furthermore, these results suggest a positive association between mental stress and dry mouth.

[背景与目的]调查口干的确切原因对于成功治疗很重要。在口干的多种原因中，精神压力被认为是唾液分泌减少的原因，尽管这一理论仍在讨论中。最近，唾液嗜铬粒蛋白A（CgA）被报道为一种新的精神压力标记物，有助于灵敏和非侵入性测量。在这项研究中，我们测量了口干患者有限唾液样本中的 CgA。 [方法]纳入40名口干患者（男6例，女34例，年龄52~81岁）和20名年龄、性别匹配的健康受试者。分别通过唾液导管和stype同时从双侧腮腺和口底表面采集唾液。收集到的唾液立即放入塑料容器中并冷冻。 CgA测量在另一家专门机构进行单盲评估。我们特意制作了一些仅由0.20ml生理盐水组成的模拟样本，并将其与提供给实验室的唾液样本混合。 [结果]每个区域采集的唾液非常有限，范围为0.05～0.40ml。仿制样品的 CgA 测量值低于 0.05 pmol/ml。 CgA 中两个腮腺之间存在良好的相关性，但腮腺和口底之间没有相关性。健康人腮腺唾液CgA浓度低于1.02 pmol/ml。 9 名口干患者的腮腺唾液 CgA 显着升高。 [结论]即使样本有限，口干患者唾液CgA也能进行有意义的测量。此外，这些结果表明精神压力和口干之间存在正相关。