Establishment of Experimental Autoimmune Inner Ear Disease Model Using New Immunological Procedures

使用新的免疫学方法建立实验性自身免疫性内耳疾病模型

• 批准号: 15591821
• 负责人: SUZUKI Masashi
• 金额: $ 2.11万
• 依托单位: Oita University (2004-2005)大分医科大学 (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591821/
• 关键词: type II collagen; CpG motif; superantigen; MRL; lpr mouse; Freund's complete adjuvant; IgG; ICAM-1; Stria vascularis; マウス; 聴性脳幹反応; Mycobacterium tuberculosis; フロインドの完全アジュバンド; 関節炎; アジュバント; lpr; 蝸牛; 自己免疫病; 難聴; コラーゲン; CpG

Mice were sensitized with (1)type II collagen, (2)CpG motif and type II collagen, or (3)Staphylococcal enterotoxin B (superantigen) and type II collagen, and examined hearing level by auditory brainstem responses. No obvious hearing loss was detected in each group. Sensitization of type II collagen might not be strong enough in our experiments because arthritis seen in each group was slight.Hearing level of MRL/MpJ-lpr/lpr (MRL/lpr) mice was examined after they were injected with (4)superantigen or (5)Freun' s complete adjuvant (FCA). Some MRL/lpr mice showed impaired hearing after the sensitization with FCA.Hematoxylin and eosin staining could not reveal obvious pathologic changes in the cochleas of the MRL/lpr mice with hearing loss. Immunostaining for IgG and ICAM 1 was observed on the endothelia of the stria vascularis vessels of the MRL/lpr mice. These findings, however, were seen not only in the mice with hearing disturbance after FCA injection, but also in the animals without hearing loss after the injection with FCA or saline. Further studies of the immune responses on the stria vascularis vessels might be important to understand the pathophysiology of the autoimmune inner ear diseases.

用（1）II型胶原蛋白、（2）CpG基序和II型胶原蛋白、或（3）葡萄球菌肠毒素B（超抗原）和II型胶原蛋白致敏小鼠，并通过听觉脑干反应检查听力水平。各组均未发现明显听力损失。在我们的实验中，II 型胶原蛋白的致敏可能不够强，因为每组中所见的关节炎都很轻微。 MRL/MpJ-lpr/lpr (MRL/lpr) 小鼠注射 (4) 超抗原或(5)弗氏完全佐剂(FCA)。部分MRL/lpr小鼠经FCA致敏后出现听力损伤。苏木精和伊红染色未能显示听力损失MRL/lpr小鼠耳蜗明显的病理变化。在 MRL/lpr 小鼠的血管纹血管内皮细胞上观察到 IgG 和 ICAM 1 的免疫染色。然而，这些发现不仅出现在注射 FCA 后出现听力障碍的小鼠中，而且还出现在注射 FCA 或盐水后没有听力损失的动物中。进一步研究血管纹血管的免疫反应对于了解自身免疫性内耳疾病的病理生理学可能很重要。

项目成果

Immunohistochemical detection of cervical lymph node micrometastases from T2N0 tongue cancer

T2N0舌癌颈部淋巴结微转移的免疫组织化学检测

• 发表时间: 2005
• 期刊: Acta Oto-Laryngologica 125
• 作者: Yoshida K.;Kashima K.;Suenaga S.;Nomi N.;Shuto J.;Suzuki M.
• 通讯作者: Suzuki M.

Dexamethasone inhibits tumor necrosis factor-alfa-induced cytokine secretion from spiral ligament fibrocytes

地塞米松抑制肿瘤坏死因子-α诱导的螺旋韧带纤维细胞分泌细胞因子

• 发表时间: 2005
• 期刊: Hearing Research (in press)
• 作者: Maeda K.;Yoshida K.;Ichimiya I.;Suzuki M.
• 通讯作者: Suzuki M.

蝸牛の免疫反応と難聴病態

耳蜗免疫反应和听力损失病理学

• 发表时间: 2004
• 期刊: 第4回鎌倉カンファレンス記録集
• 作者: 一宮一成;吉田和秀;鈴木正志
• 通讯作者: 鈴木正志

Immunohistochemical detection of cervical lymph node micrometastases from T2NO tongue cancer

T2NO舌癌颈部淋巴结微转移的免疫组化检测

• 发表时间: 2005
• 期刊: Acta Oto-Laryngologica 125
• 作者: Yoshida K.;Kashima K.;Suenaga S.;Nomi N.;Shuto J.;Suzuki M.
• 通讯作者: Suzuki M.

Dexamethasone inhibits tumor necrosis factor- a - induced cytokine secretion from spiral ligament fibrocytes

地塞米松抑制肿瘤坏死因子-a诱导的螺旋韧带纤维细胞分泌细胞因子

• 发表时间: 2005
• 期刊: Hearing Research 202
• 作者: Maeda Ke.;Yoshida K.;Ichimiya I.;Suzuki M.
• 通讯作者: Suzuki M.