Study for neuronal regeneration in ischemic brain using bone marrow transplantation

骨髓移植促进缺血脑神经元再生的研究

• 批准号: 15591555
• 负责人: KAWAMOTO Keiji
• 金额: $ 2.18万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15591555/
• 关键词: bone marrow; stem cell; transplantation; differentiation; f regeneration; Regeneration; bone marrow; ischemic brain; flow cytometry

The concept of neuronal regeneration has been rapidly changed from the past regenerative medicine. Recently neuronal differentiation and transplantation could be possible using by embryonal stem cell. Regeneration is much interested in the clinical field, so we studied for the possibility of neuronal regeneration in ischemic bran using bone marrow transplantation.Experiment of ischemic brainWe succeeded in modeling complete occulusion of ipsilatenal middle cerebral artery.Differentiation of bone marrow stem cells (BMC)BMS including the hematopoietic and mesenchymal cells could be differentiated into neuronal direction by adding PDGF-BB and/or b-FGF.Sorting and neuronal expression of CD34CD34- and CD34+ cells of lineage depleted BMC were sorted and neuronal differentiation showing lin-CD34-adult murine BMC were found by immunocytochemistry and PCR.Improvement of ischemic brainThe ischemic region was improved by transplantation of BMC.

神经元再生的概念与过去的再生医学相比已经发生了迅速的变化。最近，利用胚胎干细胞可以实现神经元分化和移植。再生在临床领域备受关注，因此我们研究了利用骨髓移植在缺血麸皮中神经元再生的可能性。缺血脑实验我们成功地建立了同侧大脑中动脉完全闭塞的模型。骨髓干细胞（BMC）的分化BMS包括造血细胞和间充质细胞，通过添加PDGF-BB和/或b-FGF可以向神经元方向分化。对谱系耗尽的BMC的CD34CD34-和CD34+细胞进行分选，并通过免疫细胞化学和PCR发现显示lin-CD34-成年小鼠BMC的神经元分化。缺血脑的改善通过移植BMC可改善缺血区域。

项目成果

Neuronal and glial differentiation following culture of the human embryonic cortical stem cells

人胚胎皮质干细胞培养后的神经元和神经胶质分化

• 发表时间: 2003
• 期刊: Human cell 16
• 作者: Peng Liang;Zhao Shiguang;Keiji Kawamoto;et al.
• 通讯作者: et al.

Neuronal and glial differentiation following culture of ES cells

ES 细胞培养后的神经元和神经胶质分化

• 发表时间: 2003
• 期刊: 16
• 作者: LIANG P;KAWAMOTO K;et al.
• 通讯作者: et al.

LIANG P, KAWAMOTO K, et al.: "Neuronal and glial differentiation following culture of ES cells"HUMAN CELL. 16・3. 151-154 (2003)

LIANG P、KAWAMOTO K 等：“ES 细胞培养后的神经元和神经胶质分化”16・3（2003 年）。