FLOWCYTOMETRIC ANALYSES OF EXPRESSIONS OF GLYCOPROTEIN ANTIGEN AND EGF-RECEPTORS, AND DNA DISTRIBUTION IN BRAIN TUMORS

脑肿瘤中糖蛋白抗原和 EGF 受体的表达以及 DNA 分布的流式细胞术分析

基本信息

批准号：
02670640
负责人：
KAWAMOTO Keiji
金额：
$ 1.34万
依托单位：
KANSAI MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670640/
关键词：
brain tumor glycoprotein EGF EGF-receptor flow cytometry oncogene,DNA-histogram EGFーR FcM DNA Glyco protein flow cytometny brain tuwor

项目摘要

1)Flowcytometric analysis of the cell cycle in brain tumors:Flow cytometry (FCM) has been an effective method to grasp biological aspects of brain tumors. Of brain tumors, we have reported how primitive ectodermal tumor cells proliferate with the advance of passages of cell culture, and have proposed a new classification of ploidy patterns.2)Flowcytometric evaluation of action mechanism of antineoplastic agents:The effects of cisplatin and carboplatin appear at the S and G_2M phase. NK cells sorted with sterile technique have antineoplastic effects and the effects could be enhanced by addition of interleukin-1. The effects of lymphokine-activated killer cells were confirmed in vitro and the action mechanism was clarified.3)Expression of oncogene products in brain tumors:In established brain tumor cell strains, immunohistochemical and flowcytometric methods were useful for evaluation of the positivity of c-myc products expression.4)Expression of glycoprotein antigen in brain tumors:The subjects included an established KB cell line, brain tumors, lung cancers, uterus carcinomas, cancers in the head and neck. Of brain tumors, the expression was highly positive in metastatic tumors and epithelial cancers. FCM was useful in evaluating the expression.5)Expressions of EGF and EGF-R:The expressions differed according to the method of sample fixation. EGF expressed in various brain tumors, suggesting a correlation with malignancy. FCM was a simple and useful method for such study.

1）脑肿瘤细胞周期的流量术分析：流式细胞术（FCM）是掌握脑肿瘤生物学方面的有效方法。在脑肿瘤中，我们报道了原始外胚性肿瘤细胞如何随细胞培养的转移的发展而增殖，并提出了对倍性模式的新分类。2）抗肿瘤剂的作用机制的流动性评估：顺铂和碳蛋白的作用出现在S和carboplatin的作用。用无菌技术排序的NK细胞具有抗塑性作用，可以通过添加白介素1来增强效果。在体外证实了淋巴运动激活的杀伤细胞的作用，并阐明了作用机制。3）癌基因产物在脑肿瘤中的表达：在既定的脑肿瘤细胞菌株中，免疫组织化学和流动性方法量表有用。肿瘤，肺癌，子宫癌，头部和颈部的癌症。在脑肿瘤中，该表达在转移性肿瘤和上皮癌中高度阳性。 FCM在评估表达式中很有用。5）EGF和EGF-R的表达式：根据样品固定方法，表达式不同。 EGF在各种脑肿瘤中表达，表明与恶性肿瘤相关。 FCM是这项研究的简单且有用的方法。