FLOWCYTOMETRIC ANALYSES OF EXPRESSIONS OF GLYCOPROTEIN ANTIGEN AND EGF-RECEPTORS, AND DNA DISTRIBUTION IN BRAIN TUMORS

脑肿瘤中糖蛋白抗原和 EGF 受体的表达以及 DNA 分布的流式细胞术分析

基本信息

批准号：
02670640
负责人：
KAWAMOTO Keiji
金额：
$ 1.34万
依托单位：
KANSAI MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670640/
关键词：
brain tumor glycoprotein EGF EGF-receptor flow cytometry oncogene,DNA-histogram EGFーR FcM DNA Glyco protein flow cytometny brain tuwor

项目摘要

1)Flowcytometric analysis of the cell cycle in brain tumors:Flow cytometry (FCM) has been an effective method to grasp biological aspects of brain tumors. Of brain tumors, we have reported how primitive ectodermal tumor cells proliferate with the advance of passages of cell culture, and have proposed a new classification of ploidy patterns.2)Flowcytometric evaluation of action mechanism of antineoplastic agents:The effects of cisplatin and carboplatin appear at the S and G_2M phase. NK cells sorted with sterile technique have antineoplastic effects and the effects could be enhanced by addition of interleukin-1. The effects of lymphokine-activated killer cells were confirmed in vitro and the action mechanism was clarified.3)Expression of oncogene products in brain tumors:In established brain tumor cell strains, immunohistochemical and flowcytometric methods were useful for evaluation of the positivity of c-myc products expression.4)Expression of glycoprotein antigen in brain tumors:The subjects included an established KB cell line, brain tumors, lung cancers, uterus carcinomas, cancers in the head and neck. Of brain tumors, the expression was highly positive in metastatic tumors and epithelial cancers. FCM was useful in evaluating the expression.5)Expressions of EGF and EGF-R:The expressions differed according to the method of sample fixation. EGF expressed in various brain tumors, suggesting a correlation with malignancy. FCM was a simple and useful method for such study.

1）脑肿瘤细胞周期的流式细胞术分析：流式细胞术（FCM）已成为掌握脑肿瘤生物学方面的有效方法。在脑肿瘤方面，我们报道了原始外胚层肿瘤细胞如何随着细胞培养传代的进行而增殖，并提出了倍性模式的新分类。2）抗肿瘤药物作用机制的流式细胞术评价：顺铂和卡铂的作用出现在S和G_2M阶段。采用无菌技术分选的 NK 细胞具有抗肿瘤作用，并且通过添加 IL-1 可以增强该作用。体外证实了淋巴因子激活杀伤细胞的作用，并阐明了作用机制。3）脑肿瘤中癌基因产物的表达：在已建立的脑肿瘤细胞株中，免疫组化和流式细胞术方法可用于评估c- myc产物的表达。4)脑肿瘤中糖蛋白抗原的表达：受试者包括已建立的KB细胞系、脑肿瘤、肺癌、子宫癌、头颈癌。在脑肿瘤中，转移性肿瘤和上皮癌中表达呈高度阳性。 FCM可用于评估表达。5)EGF和EGF-R的表达：根据样品固定方法，表达有所不同。 EGF 在各种脑肿瘤中表达，表明与恶性肿瘤相关。 FCM 是此类研究的一种简单且有用的方法。