Effect of thyroid hormone on the production of cytokines from lymphocytes

甲状腺激素对淋巴细胞产生细胞因子的影响

• 批准号: 15590487
• 负责人: HIDAKA Yoh
• 金额: $ 2.3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590487/
• 关键词: Graves' disease; thyroid hormone; Th1; Th2; IFN-γ; IL-4

The value of TSH receptor antibody will fall if thyroid function is normalized in Graves' disease. The duration of destruction is less than three months in destructive thyrotoxicosis. In order to explain these phenomenon, the hypothesis "thyroid hormone suppresses Th1 and stimulates Th2" was examined. Results ; (1) Whole blood was cultured with thyroid hormone (T3), and the amounts of IFN-gamma and IL-4 were measured with Invader RNA Assay. T3 slightly increased IFN-gamma and mildly increased IL-4. (2) Peripheral blood mononuclear cells from four people were cultured with T3, and the amounts of several cytokines were measured with TaqMan Cytokine Gene Expression Plate I. The results were different in every specimen. (3) Whole blood was cultured with PHA and T3, and the amount of IL-4 in a supernatant was measured by ELISA. IL-4 was not changed significantly by T3. (4) Peripheral blood mononuclear cells were cultured with PHA and T3, and the IFN-gamma or IL-4 producing cells were measured by ELISPOT. T3 slightly increased IFN-gamma producing cells, and slightly decreased IL-4 producing cells. Conclusions ; Thyroid hormone did not show a constant change in the Cytokine production from lymphocytes.

如果甲状腺功能在Graves疾病中标准化，则TSH受体抗体的值将下降。破坏性的持续时间不到三个月的破坏性甲状腺毒素。为了解释这些现象，检查了“甲状腺激素抑制TH1并刺激Th2”的假设。结果 ; （1）用甲状腺激素（T3）培养全血，并用Invader RNA分析测量IFN-GAMMA和IL-4的量。 T3略有增加IFN-gamma，并轻度增加IL-4。 （2）用T3培养了来自四个人的外周血单核细胞，并用Taqman细胞因子基因表达板I测量了几个细胞因子的量I。每个样品中的结果都不同。 （3）用PHA和T3培养全血，并通过ELISA测量上清液中的IL-4量。 T3没有显着更改IL-4。 （4）用PHA和T3培养外周血单核细胞，并通过ELISPOT测量IFN-GAMMA或IL-4产生的细胞。 T3略有增加IFN-gamma产生细胞，并略微降低IL-4产生细胞。结论；甲状腺激素没有显示出淋巴细胞细胞因子产生的恒定变化。

项目成果

Keiko Takeoka: "Increase in serum levels of autoantibodies after attack of seasonal allergic rhinitis in patients with Graves' disease"International Archives of Allergy and Immunology. 132・3. 268-276 (2003)

Keiko Takeoka：“格雷夫斯病患者季节性过敏性鼻炎发作后血清自身抗体水平升高”，国际过敏与免疫学档案 132・3（2003 年）。

Serum concentrations of dehydroepiandrosterone and dehydroepiandrosterone sulfate and their relation to cytokine production during and after normal pregnancy.

脱氢表雄酮和硫酸脱氢表雄酮的血清浓度及其与正常妊娠期间和之后细胞因子产生的关系。

• 发表时间: 2004
• 期刊: Clinica Chimica Acta 340
• 作者: Miyoshi;M.;Nadai;M.;Nitta;A.;Ueyama;J.;Shimizu;A.;Takagi;K.;Nabeshima;T.;Takagi;K.;Saito;K.;Hasegawa;T.;Ishida Tetsuo;Noriko Tagawa
• 通讯作者: Noriko Tagawa

Yukiko Izumi: "Relation between postpartum liver dysfunction and anti-cytochrome 2D6 antibodies"American Journal of Reproductive Immunology. 50・4. 355-362 (2003)

泉由纪子：“产后肝功能障碍与抗细胞色素 2D6 抗体的关系”美国生殖免疫学杂志 50・4（2003）。

Increase in serum levels of autoantibodies after attack of seasonal allergic rhinitis in patients with Graves' disease.

格雷夫斯病患者季节性过敏性鼻炎发作后血清自身抗体水平升高。

• 发表时间: 2003
• 期刊: International Archives of Allergy and Immunology 132・3
• 作者: Ishida Tetsuo;Tetsuo Ishida;Atsushi Hirano;Hirohisa Ichikawa;Mikio Kataoka;片岡幹男;Noriko Tagawa;Nobuyuki Amino;Keiko Takeoka
• 通讯作者: Keiko Takeoka

Nobuyuki Amino: "No increase of blocking type anti-thyrotropin receptor antibodies during pregnancy in patients with Graves' disease"Journal of Clinical Endocrinology and Metabolism. 88・12. 5871-5874 (2003)

Nobuyuki Amino：“格雷夫斯病患者妊娠期间阻断型抗促甲状腺激素受体抗体没有增加”临床内分泌与代谢杂志88・12（2003）。