Effect of thyroid stimulating hormone on the production of antibody

促甲状腺激素对抗体产生的影响

• 批准号: 17590492
• 负责人: HIDAKA Yoh
• 金额: $ 2.3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17590492/
• 关键词: TSH; TSH receptor antibody; IgG; IL-4; IL-13; Graves' disease; TSHレセプ夕ー抗体

It is the pathogenesis of Graves' disease that the stimulated type antibody (TSAb) to the receptor (TSH-R) of thyroid stimulating hormone (TSH) stimulates thyrocytes, and increases the production of thyroid hormone. We speculate that TSAb also stimulates lymphocytes through TSH-R, and promotes the production of antibody from B lymphocytes. TSAb mimics the action of TSH. Therefore the present study was performed to assess the effect of TSH on the production of antibody. Human peripheral blood mononuclear cells (PBMCs) were incubated with human TSH for seven days. Supernatant concentrations of IgG were measured. Increased levels of IgG were observed when PBMCs were incubated with 10〜100 μU/mL of TSH, matching those observed in hypothyroidism. Then we examined the effect of TSH on the production of cytokines relevant to the antibody production. However the levels of IL-4 and IL-13 were below detection sensitivity. PBMCs were subsequently incubated with TSH for seven days and exposed to PWM on the final day of incubation. A positive correlation was observed between the concentration of IgG and IL-13 (r=0.64,p<0.01), although a correlation between the concentration of IgG and IL-4 was not observed. These results suggest that TSH-stimulated IL-13 production might promote the secretion of antibody and that TSAb might induce the production of IgG, including TSAb itself.

Graves病的发病机制是促甲状腺激素（TSH）受体（TSH-R）的刺激型抗体（TSAb）刺激甲状腺细胞，增加甲状腺激素的产生。我们推测TSAb还通过刺激淋巴细胞。 TSH-R，并促进 B 淋巴细胞产生抗体，因此本研究旨在评估 TSH 对人外周血抗体产生的影响。将单核细胞 (PBMC) 与人 TSH 一起孵育 7 天，当 PBMC 与 10-100 μU/mL 的 TSH 一起孵育时，观察到 IgG 水平升高，这与甲状腺功能减退症中观察到的情况相符。 TSH 对与抗体产生相关的细胞因子产生的影响然而，随后将 PBMC 与 TSH 一起孵育 7 天，其水平低于检测灵敏度。在孵育的最后一天暴露于 PWM 中，观察到 IgG 和 IL-13 浓度之间呈正相关（r=0.64，p<0.01），但未观察到 IgG 和 IL-4 浓度之间存在相关性。这些结果表明，TSH 刺激的 IL-13 产生可能会促进抗体的分泌，而 TSAb 可能会诱导 IgG 的产生，包括 TSAb 本身。

项目成果

抗甲状腺ペルオキシダーゼ抗体(抗甲状腺マイクロゾーム抗体)

抗甲状腺过氧化物酶抗体（抗甲状腺微粒体抗体）

• 发表时间: 2005
• 期刊: medicina 42(12)
• 作者: Tateno K;et al.;日高 洋
• 通讯作者: 日高 洋

Chronic (Hashimoto's) thyroiditis. Endocrinology (5th ed.) (In : DeGroot LJ, Jameson JL eds.)

慢性（桥本氏）甲状腺炎。

• 发表时间: 2006
• 作者: Amino N;Hidaka Y
• 通讯作者: Hidaka Y

Anti-thyroglobulin antibody, Anti-thyroid peroxidase antibody

抗甲状腺球蛋白抗体、抗甲状腺过氧化物酶抗体

• 发表时间: 2005
• 期刊: Medicina 42 (12)
• 作者: Sugimori K;Matsui K;Motomura H;Tokoro T;Wang J;Kitajima I;Hidaka Y
• 通讯作者: Hidaka Y

Secretion of IL-4 and IgG from peripheral blood mononuclear cells in allergic rhinitis.

过敏性鼻炎外周血单核细胞分泌 IL-4 和 IgG。

• 期刊: Journal of Investigational Allergology and Clinical Immunology (in press)
• 作者: Hidaka Y;Tatsumi K
• 通讯作者: Tatsumi K

How to treat for Chronic thyroiditis (Hashimoto disease)?

慢性甲状腺炎（桥本病）如何治疗？

• 发表时间: 2005
• 期刊: Diagnosis and Treatment 93 (7)
• 作者: T.Kojima;(ed.by S.Asano;et al.);梅木 一美;Hidaka Y
• 通讯作者: Hidaka Y