Regulation of integrin-mediated adhesion and immune response by Rap1 and its downstream effector, p30.

Rap1 及其下游效应子 p30 调节整合素介导的粘附和免疫反应。

• 批准号: 15590436
• 负责人: KATAGIRI Koko
• 金额: $ 2.3万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590436/
• 关键词: Rap1; RAPL; Integrin; cell polarization; migration; homing; chemokine; adhesion; 生体内移動; PAPL; Bリンパ球分化; LFA-1; Tリンパ球

Immunosurveillance requires the coordinated regulation of chemokines and adhesion molecules to guide immune cell migration. However, the critical molecule to govern high trafficking capability of immune cells is not clear. We showed that RAPL plays indispensable roles in integrin-mediated adhesion and migration of lymphocytes and dendritic cells. RAPL deficiency causes defective chemokine-triggered lymphocyte adhesion and migration to secondary lymphoid organs, resulting in atrophic lymphoid follicles and deficient marginal zone B cells, concomitant with increased immature B cells in the blood. Furthermore, splenic dendritic cells were diminished and defective in adhesion. Upon activation with inflammatory stimuli, skin and splenic dendritic cells failed to migrate into either the draining lymph nodes or the white pulp of the spleen. Thus, RAPL is a crucial immune cell trafficking regulator essential for immunosurveillance.

免疫监测需要对趋化因子和粘附分子的协调调节，以引导免疫细胞迁移。但是，尚不清楚控制免疫细胞的高运输能力的关键分子。我们表明，RAPL在整联蛋白介导的粘附和淋巴细胞和树突状细胞的迁移中起着不可或缺的作用。 RAPL缺乏会导致趋化因子触发的淋巴细胞粘附和迁移到继发性淋巴机构，从而导致萎缩性淋巴卵泡和缺乏边缘区B细胞，与血液中未成熟的B细胞增加。此外，脾树突状细胞在粘附方面减少和缺陷。用炎症刺激激活后，皮肤和脾树突状细胞无法迁移到排水淋巴结或脾脏的白浆。因此，RAPL是一种至关重要的免疫细胞运输调节剂，对免疫监视必不可少。

项目成果

Rap1-mediated lymphocyte function-associated antigen-1 activation by the T cell antigen receptor is dependent on phospholipase C-gamma1.

Rap1 介导的 T 细胞抗原受体对淋巴细胞功能相关的抗原 1 的激活依赖于磷脂酶 C-gamma1。

• 发表时间: 2004
• 期刊: J Biol.Chem. 275
• 作者: Katagiri;K. et al.
• 通讯作者: K. et al.

Tohyama, Y., et al.: "The critical cytoplasmic regions of the αLβ2 integrin in Rap1-induced adhesion and migration"Molecular Biology of the Cell. 14. 2570-2582 (2003)

Tohyama, Y. 等人：“Rap1 诱导的粘附和迁移中 αLβ2 整合素的关键细胞质区域”《细胞分子生物学》14. 2570-2582 (2003)。

Rap1 translates chemokine signals to integrin activation, cell polarization, and motility across vascular endothelium under flow.

• DOI: 10.1083/jcb.200301133
• 发表时间: 2003-04-28
• 期刊: The Journal of cell biology
• 作者: Shimonaka M;Katagiri K;Nakayama T;Fujita N;Tsuruo T;Yoshie O;Kinashi T
• 通讯作者: Kinashi T

臨床免疫

临床免疫学

• 发表时间: 2003
• 作者: Shimonaka M;Katagiri K;Nakayama T;Fujita N;Tsuruo T;Yoshie O;Kinashi.T.;片桐 晃子
• 通讯作者: 片桐 晃子

The critical cytoplasmic regions of the alphaL/beta2 integrin in Rap1-induced adhesion and migration.

Rap1 诱导的粘附和迁移中 αL/β2 整合素的关键细胞质区域。

• 发表时间: 2003
• 期刊: Mol Biol Cell. 14
• 作者: Thoyama;Y.;et al.
• 通讯作者: et al.