Biological significance of fatty acid binding protein in human gastric and colonic carcinoma

脂肪酸结合蛋白在人胃癌和结肠癌中的生物学意义

基本信息

批准号：
15590308
负责人：
SUZUKI Toshimitsu
金额：
$ 2.3万
依托单位：
Fukushima Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590308/
关键词：
STOMACH CARCINOMA Colon CARCINOMA L-FATTY ACID-BINDING PROTEIN H-FATTY ACID-BINDING PROTEIN FATTY SYNTHASE LUNG CARCINOMA ENOOTHELIAL GROWTH FACTOR PROGNOSIS 浸潤転移

项目摘要

1. Liver-type fatty acid-binding protein (L-FABP) was expressed in gastric carcinoma (n=667) at 38% and lung carcinoma (n=199) at 60% in frequency, respectively. Expression of L-FABP was not associated with that of fatty acid synthase (FAS), clinicopathologic factors and patient survival in these two carcinomas. In lung carcinoma, L-FABP expression showed close association with that of vascular endothelial growth factor, suggesting possible role of L-FABP in fatty acid uptake from blood stream.2. Expression of heart-type (H-) FABP was observed at 19% in frequency in gastric carcinoma, and its expression revealed good correlation with expression of FAS, clinicopathologic factors including lymph node metastasis and hepatic metastasis, and patient survival. From these, H-FABP seems to be involved in intracellular metabolic pathway(s) of fatty acid(s) synthesized by FAS.3. L-FABP was not found in normal gastric epithelium but found at 90% in metaplastic epithelium and 38% in adenoma in frequency. Therefore L-FABP was expressed in precancerous stage of stomach carcinoma.4. In contrast with L-FABP, H-FABP was observed in neither normal nor preneoplastic (metaplasia and adenoma) gastric mucosa.5. Expression of L-FABP and H-FABP in colon carcinoma is now under investigation.6. Four colon cancer cell lines and six out of eight stomach cancer cell lines produced H-FABP, while only TKGW gastric cancer cell line produced L-FABP among 12 cell lines examined.7. By two dimensional gel electrophoresis and western blotting, three spots were formed in normal liver L-FABP, while four spots, one additional spot to those in liver L-FABP, were observed in L-FABP extracted from TKGW cells.8. Treatment with palmitate (1.9x10^<-4>- 7.5x10^<-4> mol/L) of gastric cancer cells and colon cancer cells revealed no remarkable morphological change, fat accumulation and growth retardation of cancer cells.

1. 肝脏型脂肪酸结合蛋白（L-FABP）在胃癌（n=667）和肺癌（n=199）中的表达频率分别为38%和60%。在这两种癌症中，L-FABP 的表达与脂肪酸合酶 (FAS)、临床病理因素和患者生存率无关。在肺癌中，L-FABP的表达与血管内皮生长因子的表达密切相关，提示L-FABP可能在血流脂肪酸摄取中发挥作用。2．心型（H-）FABP在胃癌中的表达频率为19%，其表达与FAS表达、临床病理因素（包括淋巴结转移和肝转移）以及患者生存具有良好的相关性。由此看来，H-FABP似乎参与了由FAS.3合成的脂肪酸的细胞内代谢途径。 L-FABP 在正常胃上皮中未发现，但在化生上皮中发现的频率为 90%，在腺瘤中发现的频率为 38%。因此L-FABP在胃癌癌前阶段有表达。4．与L-FABP相反，H-FABP在正常胃粘膜和癌前（化生和腺瘤）胃粘膜中均未观察到。5． L-FABP和H-FABP在结肠癌中的表达目前正在研究中。6．所检测的12个细胞系中，4个结肠癌细胞系和8个胃癌细胞系中的6个产生H-FABP，而只有TKGW胃癌细胞系产生L-FABP。7．二维凝胶电泳和Western blotting结果显示，正常肝脏L-FABP中形成3个斑点，TKGW细胞提取的L-FABP中形成4个斑点，比肝脏L-FABP多1个斑点。 8．棕榈酸酯(1.9x10^-4>-7.5x10^-4mol/L)处理胃癌细胞和结肠癌细胞未发现明显的形态变化、脂肪堆积和生长迟缓。