Mechanism and Inheritance of Continuous Light-Tolerance in Cucurbita moschata

西葫芦连续耐光机制及遗传

• 批准号: 15580023
• 负责人: MURAKAMI Kenji
• 金额: $ 2.11万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580023/
• 关键词: antioxidant enzyme; chlrosis; continuous light-injury; expanding leaves; mature leaves; 過酸化水素; ニホンカボチャ; 炭水化物集積

In many species, plants show injury symptoms such as chlorosis under continuous light. On the other hand, some species do not show any injury and grow well even under continuous light. In this study, we examined the mechanisms and inheritance of continuous light-tolerance using tolerant and sensitive cultivar in Cucurbita moschata.Reciprocal crosses were conducted for tolerant and sensitive cultivar. In the reciprocal F_1, the degree of chlorosis expressed as chlorophyll concentration was mid-parent value. The chlorophyll concentration of F_2 from tolerant × sensitive cultivar ranged from tolerant value to sensitive value and there was no distinct segregation. In the F_3 progeny from a sensitive F_2 plant, one sensitive and one tolerant group were segregated in approximately 9:1 ratio. These results suggested that continuous-light tolerance was controlled by two or more alleles involving recessive genes.Continuous light-injury occurred only on young, expanding leaves. We hypothesized that mature leaves sense continuous light, and this information induces the injury of new leaves. To examine this hypothesis, we covered plants partially. Injury did not occur on expanding leaves under continuous light when mature leaves were covered for 12 hr/day. Continuous light-injury did not occur on the expanding leaves of sensitive scion grafted to tolerant rootstock with mature leaves. These results indicated that the expanded leaf of sensitive cultivar produced some injury-inducer under continuous light.The activities of superoxide dismutase and catalase in continuous light-tolerant cultivar increased more rapidly than those in sensitive cultivar. These results suggested that a rapid increase in the antioxidant enzymatic activities was involved in the mechanism of tolerance to continuous light.

在许多物种中，植物在连续光下都表现出损伤症状，例如氯化。另一方面，某些物种也不会显示出任何伤害，即使在连续的光线下也会良好。在这项研究中，我们检查了使用耐受性和敏感品种在山葫芦Moschata中进行连续光耐性的机理和遗传。进行了偏跨的杂交，以耐受耐受性和敏感的品种。在相互的F_1中，叶绿素浓度表示为中间值的氯中毒程度。 F_2的叶绿素浓度从耐受×敏感的品种范围从耐受值到敏感值不等，并且没有明显的隔离。在F_3敏感F_2植物的F_3进展中，将一个灵敏的和一个耐受组的比例分离为9：1的比例。这些结果表明，连续光耐受性受到涉及隐性基因的两个或多个等位基因控制。连续的轻伤仅发生在年轻的叶子上。我们假设成熟的叶子有感觉连续光，并且这些信息引起了新叶子的伤害。为了审查这一假设，我们部分覆盖了植物。当成熟的叶子覆盖12小时/天，在连续的光线下膨胀的叶子上不会受伤。在敏感的scion叶的膨胀叶子上，没有发生连续的损伤，这些敏感的scion叶被移植到具有成熟叶子的耐受性根stock上。这些结果表明，敏感品种的叶片在连续的光线下产生了一些损伤诱导剂。超氧化物歧化酶和过氧化氢酶在连续的耐轻耐味品种中的活性比在敏感品种中的活力增长更快。这些结果表明，抗氧化剂酶活性的迅速增加与对连续光的耐受性机制有关。

项目成果

ニホンカボチャにおける連続光耐性に関連した新展開葉でのsuperoxide dismutase活性の変動

日本南瓜新发育叶片中超氧化物歧化酶活性的变化与连续耐光性相关

• 发表时间: 2004
• 期刊: 園芸学会雑誌(別冊) 73(別2)
• 作者: 村上賢治;金原行志;桝田正治
• 通讯作者: 桝田正治

村上賢治, 金原行志, 桝田正治: "ニホンカボチャにおける連続光耐性の遺伝"園芸学会雑誌(別冊). 72(別2). 365 (2003)

Kenji Murakami、Yukishi Kanehara、Masaharu Masuda：“日本南瓜的连续耐光性的继承”《园艺学会杂志》（分册）72（分册 365）（2003 年）。

カボチャ属の葉における連続光障害誘導因子は接ぎ木により伝達される

葫芦叶片中连续光损伤诱导因子通过嫁接传播

• 发表时间: 2004
• 期刊: 園芸学会雑誌(別冊) 73(別2)
• 作者: 村上賢治;浜本一恵;桝田正治
• 通讯作者: 桝田正治

村上賢治, 桝田正治: "ニホンカボチャにおける新展開葉での連続光障害発現と既展開葉との関係"園芸学会雑誌(別冊). 72(別2). 364 (2003)

Kenji Murakami、Masaharu Masuda：“日本南瓜新发育叶子中的连续光损伤表达及其与已发育叶子的关系”《园艺学会杂志》（单独卷）72（单独2）。